Overview

  • Product name

  • Description

    Rabbit Polyclonal to NKp30
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human NK-p30 aa 95-135 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    IRDVRG HDASIYVCRV EVLGLGVGTG NGTRLVVEKE HPQLG


    Database link: O14931

  • Positive control

    • Human lung carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 0.01% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20 - 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.

WB 1/100 - 1/1000. Predicted molecular weight: 22 kDa.

Target

  • Function

    Cytotoxicity-activating receptor that contributes to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation.
  • Tissue specificity

    Selectively expressed by all resting and activated NK cells and weakly expressed in spleen.
  • Sequence similarities

    Belongs to the natural cytotoxicity receptor (NCR) family.
    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1C7 antibody
    • Activating natural killer receptor p30 antibody
    • Activating NK A1 receptor antibody
    • CD337 antibody
    • CD337 antigen antibody
    • LY117 antibody
    • Lymphocyte antigen 117 antibody
    • MALS antibody
    • Natural cytotoxicity triggering receptor 3 antibody
    • Natural killer cell p30 related protein antibody
    • Natural killer cell p30-related protein antibody
    • Ncr3 antibody
    • NCTR3_HUMAN antibody
    • NK-p30 antibody
    • NKp30 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human lung carcinoma tissue, labeling NK-p30 using ab203390 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

References

ab203390 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (OV90)
Permeabilization
No
Specification
OV90
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 05 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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