Key features and details
- Mouse monoclonal [2639C1a] to NKRF
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-NKRF antibody [2639C1a]
See all NKRF primary antibodies
DescriptionMouse monoclonal [2639C1a] to NKRF
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment of human NKRF
- Purchase matching WB positive control:Recombinant Human NKRF protein
- Recombinant human NKRF.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS
Concentration information loading...
PurityProtein G purified
Purification notesab71963 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Our Abpromise guarantee covers the use of ab71963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Detects a band of approximately 40 kDa (predicted molecular weight: 78 kDa).|
|Dot blot||Use at an assay dependent dilution.|
RelevanceNKRF is a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. NKRF localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. o involved in the regulation of IL-8 transcription.
- ITBA4 antibody
- NF kappa B repressing factor antibody
- NFKB repressing factor antibody
Anti-NKRF antibody [2639C1a] (ab71963) + immunising recombinant protein
Predicted band size: 78 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
ab71963 has not yet been referenced specifically in any publications.