Key features and details
- Rabbit polyclonal to NKRF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NKRF antibody
See all NKRF primary antibodies
DescriptionRabbit polyclonal to NKRF
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Dog, Pig, Chimpanzee, Ferret, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 50-100 of Human NKRF (NP_001166959.1).
- Purchase matching WB positive control:Recombinant Human NKRF protein
- HeLa, 293 and Jurkat whole cell lysate (ab7899).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab168829 was affinity purified using an epitope specific to NKRF immobilized on solid support.
Our Abpromise guarantee covers the use of ab168829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 78 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceNKRF is a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. NKRF localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. o involved in the regulation of IL-8 transcription.
- ITBA4 antibody
- NF kappa B repressing factor antibody
- NFKB repressing factor antibody
All lanes : Anti-NKRF antibody (ab168829) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 78 kDa
Exposure time: 30 seconds
Detection of NKRF in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168829 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab168829 has been referenced in 1 publication.
- Li X et al. miR-301a promotes lung tumorigenesis by suppressing Runx3. Mol Cancer 18:99 (2019). PubMed: 31122259