Key features and details
- Rabbit polyclonal to NKRF
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NKRF antibody
See all NKRF primary antibodies
DescriptionRabbit polyclonal to NKRF
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
- Purchase matching WB positive control:Recombinant Human NKRF protein
- Human fetal muscle lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab83977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 78 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
RelevanceNKRF is a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. NKRF localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. o involved in the regulation of IL-8 transcription.
- ITBA4 antibody
- NF kappa B repressing factor antibody
- NFKB repressing factor antibody
Anti-NKRF antibody (ab83977) at 1 µg/ml (5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa
Additional bands at: ~80 kDa, ~45 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling NKRF with ab83977 at 5.0µg/ml.
ab83977 has been referenced in 1 publication.
- Zhong M et al. Malignant Transformation of Human Bronchial Epithelial Cells Induced by Arsenic through STAT3/miR-301a/SMAD4 Loop. Sci Rep 8:13291 (2018). PubMed: 30185897