Key features and details
- Rabbit polyclonal to Nkx2.2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nkx2.2 antibody
See all Nkx2.2 primary antibodies
DescriptionRabbit polyclonal to Nkx2.2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- WB: U-138 MG cell lysate; Nkx2.2 overexpression HEK-293T cell lysate. IHC-P: Human pancreas, cerebral cortex and cerebellum tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 30 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
Sequence similaritiesBelongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain.
DomainThe homeodomain is essential for interaction with OLIG2.
- Information by UniProt
- Homeobox protein NK 2 homolog B antibody
- Homeobox protein NK-2 homolog B antibody
- Homeobox protein Nkx 2.2 antibody
Anti-Nkx2.2 antibody (ab244270) at 0.4 µg/ml + U-138 MG (human glioblastoma cell line) cell lysate
Predicted band size: 30 kDa
Formalin-fixed, paraffin-embedded human pancreas tissue stained for Nkx2 with ab244270 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemical staining of human cerebellum shows moderate nuclear positivity in cells in molecular layer.
Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for Nkx2 with ab244270 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human skeletal muscle tissue shows absence of immunoreactivity for Nkx2 when incubated with ab244270 at a 1/50 dilution (negative control).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244270 has not yet been referenced specifically in any publications.