Product nameAnti-Nkx2.2 antibody
See all Nkx2.2 primary antibodies
DescriptionRabbit polyclonal to Nkx2.2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
3 synthetic peptides (Human) conjugated to KLH
- Recombinant protein produced in an induced culture of E.coli.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituent: Whole serum
Our Abpromise guarantee covers the use of ab86024 in the following tested applications.
|WB||1/1000. Predicted molecular weight: 30 kDa.|
FunctionMay be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
Sequence similaritiesBelongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain.
DomainThe homeodomain is essential for interaction with OLIG2.
- Information by UniProt
- Homeobox protein NK 2 homolog B antibody
- Homeobox protein NK-2 homolog B antibody
- Homeobox protein Nkx 2.2 antibody
All lanes : Anti-Nkx2.2 antibody (ab86024) at 1/1000 dilution
Lane 1 : An uninduced (negative control) culture of E.coli
Lane 2 : An induced culture of E.coli
All lanes : Anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 35 kDa, 37 kDa, 42 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 10%Predicted molecular weight of recombinant Nkx2.2 protein:44.1kDa (30.1kDa + another 14kDa for the tag).
ab86024 has not yet been referenced specifically in any publications.