Overview

  • Product name

  • Description

    Rabbit polyclonal to Nkx2.2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    3 synthetic peptides (Human) conjugated to KLH

  • Positive control

    • Recombinant protein produced in an induced culture of E.coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab86024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 30 kDa.

Target

  • Function

    May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
  • Sequence similarities

    Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Domain

    The homeodomain is essential for interaction with OLIG2.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homeobox protein NK 2 homolog B antibody
    • Homeobox protein NK-2 homolog B antibody
    • Homeobox protein Nkx 2.2 antibody
    • Homeobox protein Nkx-2.2 antibody
    • NK 2 homolog B antibody
    • NK2 homeobox 2 antibody
    • NK2 transcription factor like protein B antibody
    • NK2 transcription factor related locus 2 antibody
    • NKX2 2 antibody
    • NKX2-2 antibody
    • NKX22 antibody
    • NKX22_HUMAN antibody
    • Nkx2b antibody
    • tinman antibody
    see all

Images

  • All lanes : Anti-Nkx2.2 antibody (ab86024) at 1/1000 dilution

    Lane 1 : An uninduced (negative control) culture of E.coli
    Lane 2 : An induced culture of E.coli

    Secondary
    All lanes : Anti-Rabbit IgG HRP at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 130 kDa, 35 kDa, 37 kDa, 42 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration 10%

    Predicted molecular weight of recombinant Nkx2.2 protein:44.1kDa (30.1kDa + another 14kDa for the tag).

References

ab86024 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry to confirm that as far as we are aware, ab86024 Anti-Nkx2.2 antibody has not yet been tested in other applications, it has been tested in WB only. All tested and guaranteed applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention. I am sorry there are no other suitably tested antibodies to suggest on this occasion. If you would like to test the antibody in another application, please let me know prior to the purchase by replying to this message as you may be eligible for our testing discount program. Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

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