Anti-Nkx2.2 antibody (ab93188)
Key features and details
- Rabbit polyclonal to Nkx2.2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Nkx2.2 antibody
See all Nkx2.2 primary antibodies -
Description
Rabbit polyclonal to Nkx2.2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Mouse Nkx2.2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab123823) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Spinal Cord; Mouse Brain; Mouse Cerebellum; Mouse Hippocampus; Mouse Forebrain.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab93188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. -
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Domain
The homeodomain is essential for interaction with OLIG2. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 18088 Mouse
- Entrez Gene: 366214 Rat
- SwissProt: P42586 Mouse
- Unigene: 330639 Mouse
- Unigene: 32651 Rat
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Alternative names
- Homeobox protein NK 2 homolog B antibody
- Homeobox protein NK-2 homolog B antibody
- Homeobox protein Nkx 2.2 antibody
see all
Images
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Western blot - Anti-Nkx2.2 antibody (ab93188)All lanes : Anti-Nkx2.2 antibody (ab93188) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Cerebellum Mouse Tissue Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lane 5 : Mouse Hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Datasheets and documents
References (0)
ab93188 has not yet been referenced specifically in any publications.
