Recombinant Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
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Overview
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Product name
Anti-Nkx2.2 antibody [EPR14638] - N-terminal
See all Nkx2.2 primary antibodies -
Description
Rabbit monoclonal [EPR14638] to Nkx2.2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Nkx2.2 aa 1-150 (N terminal). The exact sequence is proprietary.
Database link: O95096 -
Positive control
- Human Ewing sarcoma, Human colon, Mouse brain and Rat colon tissues; C6 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14638 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab191077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/1000. | |
| ICC/IF | 1/50. |
Target
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Function
May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. -
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Domain
The homeodomain is essential for interaction with OLIG2. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4821 Human
- Entrez Gene: 18088 Mouse
- Entrez Gene: 366214 Rat
- Omim: 604612 Human
- SwissProt: O95096 Human
- SwissProt: P42586 Mouse
- Unigene: 516922 Human
- Unigene: 330639 Mouse
see all -
Alternative names
- Homeobox protein NK 2 homolog B antibody
- Homeobox protein NK-2 homolog B antibody
- Homeobox protein Nkx 2.2 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)](https://www.abcam.com/ps/products/191/ab191077/Images/ab191077-228170-anti-nkx22-antibody-epr14638-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)Immunofluorescent analysis of 4% paraformaldehyde-fixed C6 cells labeling Nkx2.2 with ab191077 at 1/50, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue). Negative controls also shown.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)](https://www.abcam.com/ps/products/191/ab191077/Images/ab191077-228169-anti-nkx22-antibody-epr14638-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)](https://www.abcam.com/ps/products/191/ab191077/Images/ab191077-228168-anti-nkx22-antibody-epr14638-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)](https://www.abcam.com/ps/products/191/ab191077/Images/ab191077-228167-anti-nkx22-antibody-epr14638-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)](https://www.abcam.com/ps/products/191/ab191077/Images/ab191077-228166-anti-nkx22-antibody-epr14638-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
Datasheets and documents
References
ab191077 has not yet been referenced specifically in any publications.
