Recombinant Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
![Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)](https://www.abcam.com/ps/products/191/ab191077/Images/ab191077-228170-anti-nkx22-antibody-epr14638-n-terminal-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14638] to Nkx2.2 - N-terminal
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Nkx2.2 antibody [EPR14638] - N-terminal
See all Nkx2.2 primary antibodies -
Description
Rabbit monoclonal [EPR14638] to Nkx2.2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Nkx2.2 aa 1-150 (N terminal). The exact sequence is proprietary.
Database link: O95096 -
Positive control
- Human Ewing sarcoma, Human colon, Mouse brain and Rat colon tissues; C6 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14638 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab191077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| ICC/IF | 1/50. |
Target
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Function
May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. -
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Domain
The homeodomain is essential for interaction with OLIG2. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4821 Human
- Entrez Gene: 18088 Mouse
- Entrez Gene: 366214 Rat
- Omim: 604612 Human
- SwissProt: O95096 Human
- SwissProt: P42586 Mouse
- Unigene: 516922 Human
- Unigene: 330639 Mouse
see all -
Alternative names
- Homeobox protein NK 2 homolog B antibody
- Homeobox protein NK-2 homolog B antibody
- Homeobox protein Nkx 2.2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
Immunofluorescent analysis of 4% paraformaldehyde-fixed C6 cells labeling Nkx2.2 with ab191077 at 1/50, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue). Negative controls also shown.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)](https://www.abcam.com/ps/products/191/ab191077/Images/ab191077-228169-anti-nkx22-antibody-epr14638-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)](https://www.abcam.com/ps/products/191/ab191077/Images/ab191077-228168-anti-nkx22-antibody-epr14638-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)](https://www.abcam.com/ps/products/191/ab191077/Images/ab191077-228167-anti-nkx22-antibody-epr14638-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)](https://www.abcam.com/ps/products/191/ab191077/Images/ab191077-228166-anti-nkx22-antibody-epr14638-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)](https://www.abcam.com/ps/products/191/ab191077/Images/ab191077-6-benefits-of-recombinant-antibodies.png)
Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
Protocols
Datasheets and documents
Certificate of Compliance
References (2)
ab191077 has been referenced in 2 publications.
- Bradley RA et al. Regionally specified human pluripotent stem cell-derived astrocytes exhibit different molecular signatures and functional properties. Development 146:N/A (2019). PubMed: 31189664
- Lam CJ et al. Highly Proliferative a-Cell-Related Islet Endocrine Cells in Human Pancreata. Diabetes 67:674-686 (2018). PubMed: 29326366
