Overview

  • Product name

    Anti-Nkx2.2 antibody [EPR14638] - N-terminal
    See all Nkx2.2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14638] to Nkx2.2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Nkx2.2 aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: O95096

  • Positive control

    • Human Ewing sarcoma, Human colon, Mouse brain and Rat colon tissues; C6 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/50.

Target

  • Function

    May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
  • Sequence similarities

    Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Domain

    The homeodomain is essential for interaction with OLIG2.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homeobox protein NK 2 homolog B antibody
    • Homeobox protein NK-2 homolog B antibody
    • Homeobox protein Nkx 2.2 antibody
    • Homeobox protein Nkx-2.2 antibody
    • NK 2 homolog B antibody
    • NK2 homeobox 2 antibody
    • NK2 transcription factor like protein B antibody
    • NK2 transcription factor related locus 2 antibody
    • NKX2 2 antibody
    • NKX2-2 antibody
    • NKX22 antibody
    • NKX22_HUMAN antibody
    • Nkx2b antibody
    • tinman antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed C6 cells labeling Nkx2.2 with ab191077 at 1/50, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue). Negative controls also shown.

  • Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

References

ab191077 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (neural tube of mouse embryos)
Permeabilization
Yes - 0.1% Triton X-100
Specification
neural tube of mouse embryos
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 31 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryo/ adult brain)
Permeabilization
Yes - 0.3% triton in PBS
Specification
embryo/ adult brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 23°C
Fixative
Paraformaldehyde

Dr. steven wyler

Verified customer

Submitted Sep 24 2015

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