Anti-Nkx2.2 antibody [NX2/294] - BSA and Azide free (ab212686)
Key features and details
- Mouse monoclonal [NX2/294] to Nkx2.2 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Nkx2.2 antibody [NX2/294] - BSA and Azide free
See all Nkx2.2 primary antibodies -
Description
Mouse monoclonal [NX2/294] to Nkx2.2 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Nkx2.2 aa 1 to the C-terminus.
Database link: O95096 -
Positive control
- Pancreas or Ewing's sarcoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
NX2/294 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
for 30 minutes at room temperature. |
Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. for 30 minutes at room temperature. |
Target
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Function
May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. -
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Domain
The homeodomain is essential for interaction with OLIG2. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4821 Human
- Entrez Gene: 366214 Rat
- Omim: 604612 Human
- SwissProt: O95096 Human
- Unigene: 516922 Human
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Alternative names
- Homeobox protein NK 2 homolog B antibody
- Homeobox protein NK-2 homolog B antibody
- Homeobox protein Nkx 2.2 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Ewing's Sarcoma tissue section labeling Nkx2.2 with ab212686 at 1 µg/mL.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue section labeling Nkx2.2 with ab212686 at 1 µg/mL.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat pancreas tissue section labeling Nkx2.2 with ab212686 at 1 µg/mL.
Datasheets and documents
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Datasheet download
References (0)
ab212686 has not yet been referenced specifically in any publications.