Anti-Nkx2.2 antibody [NX2/294] - BSA and Azide free (ab212686)

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Overview

  • Product name

    Anti-Nkx2.2 antibody [NX2/294] - BSA and Azide free
    See all Nkx2.2 primary antibodies

  • Description

    Mouse monoclonal [NX2/294] to Nkx2.2 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human

  • Immunogen

    Recombinant full length protein corresponding to Human Nkx2.2 aa 1-273.
    Sequence:

    MSLTNTKTGFSVKDILDLPDTNDEEGSVAEGPEEENEGPEPAKRAGPLGQ GALDAVQSLPLKNPFYDSSDNPYTRWLASTEGLQYSLHGLAAGAPPQDSS SKSPEPSADESPDNDKETPGGGGDAGKKRKRRVLFSKAQTYELERRFRQQ RYLSAPEREHLASLIRLTPTQVKIWFQNHRYKMKRARAEKGMEVTPLPSP RRVAVPVLVRDGKPCHALKAQDLAAATFQAGIPFSAYSAQSLQHMQYNAQ YSSASTPQYPTAHPLVQAQQWTW


    Database link: O95096

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Pancreas or Ewing's sarcoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab212686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 106 cells.

Final volume 100 µL.
ICC/IF Use a concentration of 1 - 2 µg/ml.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

for 30 minutes at room temperature.

Target

  • Function

    May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.

  • Sequence similarities

    Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • Domain

    The homeodomain is essential for interaction with OLIG2.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Homeobox protein NK 2 homolog B antibody
    • Homeobox protein NK-2 homolog B antibody
    • Homeobox protein Nkx 2.2 antibody
    • Homeobox protein Nkx-2.2 antibody
    • NK 2 homolog B antibody
    • NK2 homeobox 2 antibody
    • NK2 transcription factor like protein B antibody
    • NK2 transcription factor related locus 2 antibody
    • NKX2 2 antibody
    • NKX2-2 antibody
    • NKX22 antibody
    • NKX22_HUMAN antibody
    • Nkx2b antibody
    • tinman antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] - BSA and Azide free (ab212686)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] - BSA and Azide free (ab212686)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Ewing's Sarcoma tissue section labeling Nkx2.2 with ab212686 at 1 µg/mL.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] - BSA and Azide free (ab212686)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] - BSA and Azide free (ab212686)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue section labeling Nkx2.2 with ab212686 at 1 µg/mL.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] - BSA and Azide free (ab212686)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] - BSA and Azide free (ab212686)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat pancreas tissue section labeling Nkx2.2 with ab212686 at 1 µg/mL.

References

ab212686 has not yet been referenced specifically in any publications.

Publishing research using ab212686? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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CyTOF abreview for Anti-Nkx2.2 antibody [NX2/294] - BSA and Azide free

 Excellent
Abreviews
Application
CyTOF®
Sample
Mouse Cell (Lung)
Specification
Lung
Read More

Irit Milman Krentsis

Verified customer

Submitted Nov 14 2017

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