Overview

  • Product name

  • Description

    Rabbit polyclonal to NKX2.3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Guinea pig, Cow
  • Immunogen

    Synthetic peptide corresponding to region within C terminal amino acids 311-330 (AAAYSGSYGC AYPTGGGGGG GGTASAATTA MQPACSATGG GSFVNVSNLG) of Mouse NKX2.3 (NP_032725).

  • Positive control

    • Mouse uterus lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab102040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Relevance

    This protein belongs to the NKX family of homeodomain containing transcription factors. These are ivolveded in cell type specification and in the maintenance of differentiated tissue functions.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Homeobox protein NK-2 homolog C antibody
    • homeobox protein NKX2-3 homolog antibody
    • MGC127415 antibody
    • NK-2 (Drosophila) homolog C antibody
    • NK2 transcription factor homolog C antibody
    • NK2 transcription factor related, locus 3 antibody
    • NK2.3 antibody
    • NKX2.3 antibody
    • NKX23 antibody
    • NKX2C antibody
    • tinman antibody
    see all

Images

  • Anti-NKX2.3 antibody (ab102040) at 0.25 µg/ml + Mouse uterus lysate at 10 µg

    Predicted band size: 38 kDa



    12% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen lymphoid tissue labelling NKX2.3 with ab102040 at 5µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lymphoid tissue (skeletal muscle) labelling NKX2.3 with ab102040 at 5µg/ml.

References

ab102040 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
Western blot
Sample
Human Cell lysate - whole cell (Lane 1: hu retinal endothelial cells (EC), Lane 2:)
Gel Running Conditions
Non-reduced Denaturing (4-15% precast polyacrylamide gel)
Loading amount
25 µg
Specification
Lane 1: hu retinal endothelial cells (EC), Lane 2:
Blocking step
SEA BLOCK Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Andrea Cabrera

Verified customer

Submitted Mar 19 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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