Western blot abreview for Anti-NKX2.3 antibody

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Lane 1: hu retinal endothelial cells (EC), Lane 2:)
Gel Running Conditions
Non-reduced Denaturing (4-15% precast polyacrylamide gel)
Loading amount
25 µg
Specification
Lane 1: hu retinal endothelial cells (EC), Lane 2:
Blocking step
SEA BLOCK Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Concentration
1 µg/ml

Secondary antibody

Concentration
0.25 µg/ml
Name
Non-Abcam antibody was used: was DyLight 800 Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Other

Detection

Detection method
Fluorescence
Exposure
2 minute(s) and 30 second(s)
Bands
Specific: 38 kDa Non-specific: 250, 60, 20 kDa

Andrea Cabrera

Verified customer

Submitted Mar 19 2019

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