Overview

  • Product name

  • Description

    Rabbit polyclonal to NKX2.3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: EHHFHSAPCM LAAAEGTQFS DGGEEDEEDE GEKLSYLNSL AAADGHGDSG, corresponding to N terminal amino acids 37-86 of Human NKX2.3

  • Positive control

    • Fetal spleen lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • Relevance

    This protein belongs to the NKX family of homeodomain containing transcription factors. These are ivolveded in cell type specification and in the maintenance of differentiated tissue functions.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Homeobox protein NK-2 homolog C antibody
    • homeobox protein NKX2-3 homolog antibody
    • MGC127415 antibody
    • NK-2 (Drosophila) homolog C antibody
    • NK2 transcription factor homolog C antibody
    • NK2 transcription factor related, locus 3 antibody
    • NK2.3 antibody
    • NKX2.3 antibody
    • NKX23 antibody
    • NKX2C antibody
    • tinman antibody
    see all

Images

  • Anti-NKX2.3 antibody (ab66366) at 0.25 µg/ml + Fetal spleen lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa
    Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.

References

ab66366 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Ms retina, Rat retina, Ms Spleen, Ms skeletal mus)
Gel Running Conditions
Non-reduced Denaturing (4-15% precast polyacrylamide gel)
Loading amount
25 µg
Specification
Ms retina, Rat retina, Ms Spleen, Ms skeletal mus
Blocking step
SEA BLOCK Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 19 2019

Answer

Thank you for your inquiry.

We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

I have conducted a search for you and found the following antibodies:

FOXJ2 - 2 antibodies but not tested yet in Rat (suitable for Western blot)

ab22857, ab49160

HAND1 - 4 antibodies but not tested yet in Rat (suitable for Western blot)

ab46822, ab52767, ab86009, ab104320



NFAT5

ab3446 - reacts with Rat

ab105886 - predicted to react with Rat





Nkx2-3

ab49162 - predicted to react with Rat



ab66366- predicted to react with Rat





Nkx2-4

ab48331 - predicted to react with Rat



ab49162 - predicted to react with Rat



ab66366 - predicted to react with Rat


If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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