Key features and details
- Goat polyclonal to Nkx2.5
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Nkx2.5 antibody
See all Nkx2.5 primary antibodies
DescriptionGoat polyclonal to Nkx2.5
Specificityab106923 is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog
- This antibody gave a positive signal in the following whole cell lysates: Human fetal heart; Mouse heart; Rat Heart
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab106923 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab106923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa).
A 1 hour primary incubation is recommended for this product. Abcam recommends using 3% milk as the blocking agent.
FunctionImplicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
Tissue specificityExpressed only in the heart.
Involvement in diseaseAtrial septal defect 7, with or without atrioventricular conduction defects
Tetralogy of Fallot
Conotruncal heart malformations
Hypothyroidism, congenital, non-goitrous, 5
Ventricular septal defect 3
Hypoplastic left heart syndrome 2
Asplenia, isolated congenital
Sequence similaritiesBelongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- NKX2.5, mouse, homolog of antibody
- Cardiac-specific homeobox 1 antibody
- Cardiac-specific homeobox antibody
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton staining Nkx2.5. Primary incubation with ab51983 (5µg/ml) for 1 hour followed by Alexa Fluor 488 secondary antibody (2µg/ml). Nuclear counter stain is DAPI.
Anti-Nkx2.5 antibody (ab106923) at 0.3 µg/ml + Human heart lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Primary incubation was 1 hour.
The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684).
All lanes : Anti-Nkx2.5 antibody (ab106923) at 1 µg/ml (Blocked with 3% Milk)
Lane 1 : Human Nkx2.5 recombinant protein (tagged) at 0.1 µg
Lane 2 : HEK293 (Human) Whole Cell Lysate (ab52256) at 20 µg
Lane 3 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate (negative control) at 20 µg
Lane 4 : Heart (Mouse) Mouse Tissue Lysate at 20 µg
Lane 5 : Heart (Rat) Tissue Lysate at 20 µg
Lane 6 : Human Fetal Heart - Total Protein Tissue Lysate at 20 µg
All lanes : Rabbit Anti-Goat IgG H&L (HRP) (ab97100) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab106923 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab106923 has been referenced in 4 publications.
- Chatterjee S & Chakravarti A A gene regulatory network explains RET-EDNRB epistasis in Hirschsprung disease. Hum Mol Genet 28:3137-3147 (2019). PubMed: 31313802
- Chen J et al. Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL-1 cells. Exp Ther Med 18:4631-4636 (2019). PubMed: 31798700
- Liu H et al. Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis. Stem Cells Int 2016:2524092 (2016). PubMed: 26798354
- Mohammadi A et al. Generation of Rat Embryonic Germ Cells via Inhibition of TGFß and MEK Pathways. Cell J 17:288-95 (2015). Rat . PubMed: 26199907