• Product name
  • Description
    Goat polyclonal to Nkx2.5
  • Host species
  • Specificity
    ab106923 is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 94-107 of Human Nkx2.5 (NP_004378.1; NP_001159647.1; NP_001159648.1).

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Human fetal heart; Mouse heart; Rat Heart
  • General notes



Our Abpromise guarantee covers the use of ab106923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa).

Abcam recommends using 3% milk as the blocking agent.


  • Function
    Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
  • Tissue specificity
    Expressed only in the heart.
  • Involvement in disease
    Atrial septal defect 7, with or without atrioventricular conduction defects
    Tetralogy of Fallot
    Conotruncal heart malformations
    Hypothyroidism, congenital, non-goitrous, 5
    Ventricular septal defect 3
    Hypoplastic left heart syndrome 2
    Asplenia, isolated congenital
  • Sequence similarities
    Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • NKX2.5, mouse, homolog of antibody
    • Cardiac-specific homeobox 1 antibody
    • Cardiac-specific homeobox antibody
    • CHNG5 antibody
    • CSX antibody
    • CSX1 antibody
    • FLJ52202 antibody
    • FLJ97166 antibody
    • FLJ97195 antibody
    • FLJ97197 antibody
    • FLJ99536 antibody
    • HLHS2 antibody
    • Homeobox protein CSX antibody
    • Homeobox protein NK-2 homolog E antibody
    • Homeobox protein Nkx 2.5 antibody
    • Homeobox protein Nkx-2.5 antibody
    • NK2 homeobox 5 antibody
    • NK2 transcription factor related locus 5 antibody
    • NK2 transcription factor related, locus 5 (Drosophila) antibody
    • NK2, Drosophila, homolog of, E antibody
    • NKX2-5 antibody
    • NKX2.5 antibody
    • NKX25_HUMAN antibody
    • Nkx2E antibody
    • NKX4-1 antibody
    • Tinman paralog antibody
    • VSD3 antibody
    see all


  • Immunofluorescence analysis of paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton staining Nkx2.5. Primary incubation with ab51983 (5µg/ml) for 1 hour followed by Alexa Fluor 488 secondary antibody (2µg/ml). Nuclear counter stain is DAPI.
  • Anti-Nkx2.5 antibody (ab106923) at 0.3 µg/ml + Human heart lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 35 kDa

    Primary incubation was 1 hour.

    The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684).
  • All lanes : Anti-Nkx2.5 antibody (ab106923) at 1 µg/ml (Blocked with 3% Milk)

    Lane 1 : Human Nkx2.5 recombinant protein (tagged) at 0.1 µg
    Lane 2 : HEK293 (Human) Whole Cell Lysate (ab52256) at 20 µg
    Lane 3 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate (negative control) at 20 µg
    Lane 4 : Heart (Mouse) Mouse Tissue Lysate at 20 µg
    Lane 5 : Heart (Rat) Tissue Lysate at 20 µg
    Lane 6 : Human Fetal Heart - Total Protein Tissue Lysate at 20 µg

    All lanes : Rabbit Anti-Goat IgG H&L (HRP) (ab97100) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 45 kDa (why is the actual band size different from the predicted?)

    Exposure time: 4 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab106923 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.


This product has been referenced in:
  • Liu H  et al. Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis. Stem Cells Int 2016:2524092 (2016). Read more (PubMed: 26798354) »
  • Mohammadi A  et al. Generation of Rat Embryonic Germ Cells via Inhibition of TGFß and MEK Pathways. Cell J 17:288-95 (2015). Rat . Read more (PubMed: 26199907) »

See all 2 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Cell lysate - whole cell (H9C2 cell line)
Loading amount
150000 cells
H9C2 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Maria Angela D'Amico

Verified customer

Submitted Dec 16 2011


Sign up