Product nameAnti-Nkx2.5 antibody [EPR20168]
See all Nkx2.5 primary antibodies
DescriptionRabbit monoclonal [EPR20168] to Nkx2.5
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Nkx2.5. The exact sequence is proprietary.
Database link: P52952
- WB: Human fetal heart muscle tissue lysate; HEK-293T and HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab205263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 30-42 kDa (predicted molecular weight: 35 kDa).|
FunctionImplicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
Tissue specificityExpressed only in the heart.
Involvement in diseaseAtrial septal defect 7, with or without atrioventricular conduction defects
Tetralogy of Fallot
Conotruncal heart malformations
Hypothyroidism, congenital, non-goitrous, 5
Ventricular septal defect 3
Hypoplastic left heart syndrome 2
Asplenia, isolated congenital
Sequence similaritiesBelongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- NKX2.5, mouse, homolog of antibody
- Cardiac-specific homeobox 1 antibody
- Cardiac-specific homeobox antibody
All lanes : Anti-Nkx2.5 antibody [EPR20168] (ab205263) at 1/1000 dilution
Lane 1 : Human fetal heart muscle tissue lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 30-42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking/Dilution: 5% NFDM/TBST
The molecular masses observed are consistent with what has been described in the literature (PMID: 21447557)
Negative control: MCF7 (PMID: 15340050).
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
ab205263 has not yet been referenced specifically in any publications.