Recombinant Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)
![Western blot - Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)](https://www.abcam.com/ps/products/228/ab228335/Images/ab228335-291686-anti-nkx25-antibody-epr20168-western-blot.jpg "Western blot - Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20168] to Nkx2.5 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free
See all Nkx2.5 primary antibodies -
Description
Rabbit monoclonal [EPR20168] to Nkx2.5 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Nkx2.5. The exact sequence is proprietary.
Database link: P52952 -
Positive control
- WB: Human fetal heart muscle tissue lysate; HEK-293T and HeLa whole cell lysate.
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General notes
Ab228335 is the carrier-free version of ab205263. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab228335 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20168 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab228335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 30-42 kDa (predicted molecular weight: 35 kDa). |
Target
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Function
Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development. -
Tissue specificity
Expressed only in the heart. -
Involvement in disease
Atrial septal defect 7, with or without atrioventricular conduction defects
Tetralogy of Fallot
Conotruncal heart malformations
Hypothyroidism, congenital, non-goitrous, 5
Ventricular septal defect 3
Hypoplastic left heart syndrome 2
Asplenia, isolated congenital -
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1482 Human
- Omim: 600584 Human
- SwissProt: P52952 Human
- Unigene: 54473 Human
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Alternative names
- NKX2.5, mouse, homolog of antibody
- Cardiac-specific homeobox 1 antibody
- Cardiac-specific homeobox antibody
see all
Images
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Western blot - Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)All lanes : Anti-Nkx2.5 antibody [EPR20168] (ab205263) at 1/1000 dilution
Lane 1 : Human fetal heart muscle tissue lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 30-42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution: 5% NFDM/TBST
The molecular masses observed are consistent with what has been described in the literature (PMID: 21447557)
Negative control: MCF7 (PMID: 15340050).
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205263).
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![Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)](https://www.abcam.com/ps/products/228/ab228335/Images/ab228335-2-benefits-of-recombinant-antibodies.png)
Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab228335 has not yet been referenced specifically in any publications.
