Recombinant
RabMAb

Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)

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Overview

  • Product name
    Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free
    See all Nkx2.5 primary antibodies
  • Description
    Rabbit monoclonal [EPR20168] to Nkx2.5 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Nkx2.5. The exact sequence is proprietary.
    Database link: P52952

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • WB: Human fetal heart muscle tissue lysate; HEK-293T and HeLa whole cell lysate.
  • General notes

    ab228335 is a PBS-only buffer format of ab205263. Please refer to ab205263 for recommended dilutions, protocols, and image data.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab228335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 30-42 kDa (predicted molecular weight: 35 kDa).

Target

  • Function
    Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
  • Tissue specificity
    Expressed only in the heart.
  • Involvement in disease
    Atrial septal defect 7, with or without atrioventricular conduction defects
    Tetralogy of Fallot
    Conotruncal heart malformations
    Hypothyroidism, congenital, non-goitrous, 5
    Ventricular septal defect 3
    Hypoplastic left heart syndrome 2
    Asplenia, isolated congenital
  • Sequence similarities
    Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • NKX2.5, mouse, homolog of antibody
      • Cardiac-specific homeobox 1 antibody
      • Cardiac-specific homeobox antibody
      • CHNG5 antibody
      • CSX antibody
      • CSX1 antibody
      • FLJ52202 antibody
      • FLJ97166 antibody
      • FLJ97195 antibody
      • FLJ97197 antibody
      • FLJ99536 antibody
      • HLHS2 antibody
      • Homeobox protein CSX antibody
      • Homeobox protein NK-2 homolog E antibody
      • Homeobox protein Nkx 2.5 antibody
      • Homeobox protein Nkx-2.5 antibody
      • NK2 homeobox 5 antibody
      • NK2 transcription factor related locus 5 antibody
      • NK2 transcription factor related, locus 5 (Drosophila) antibody
      • NK2, Drosophila, homolog of, E antibody
      • NKX2-5 antibody
      • NKX2.5 antibody
      • NKX25_HUMAN antibody
      • Nkx2E antibody
      • NKX4-1 antibody
      • Tinman paralog antibody
      • VSD3 antibody
      see all

    Images

    • Western blot - Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)
      Western blot - Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)
      All lanes : Anti-Nkx2.5 antibody [EPR20168] (ab205263) at 1/1000 dilution

      Lane 1 : Human fetal heart muscle tissue lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 35 kDa
      Observed band size: 30-42 kDa (why is the actual band size different from the predicted?)


      Exposure time: 3 minutes


      Blocking/Dilution: 5% NFDM/TBST

      The molecular masses observed are consistent with what has been described in the literature (PMID: 21447557)

      Negative control: MCF7 (PMID: 15340050).

      The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205263).

    References

    ab228335 has not yet been referenced specifically in any publications.

    Publishing research using ab228335? Please let us know so that we can cite the reference in this datasheet.

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