The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Detects a band of approximately 30-42 kDa (predicted molecular weight: 35 kDa).
Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
Expressed only in the heart.
Involvement in disease
Atrial septal defect 7, with or without atrioventricular conduction defects Tetralogy of Fallot Conotruncal heart malformations Hypothyroidism, congenital, non-goitrous, 5 Ventricular septal defect 3 Hypoplastic left heart syndrome 2 Asplenia, isolated congenital
Belongs to the NK-2 homeobox family. Contains 1 homeobox DNA-binding domain.
NK2 transcription factor related, locus 5 (Drosophila) antibody
NK2, Drosophila, homolog of, E antibody
Tinman paralog antibody
Western blot - Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)
All lanes : Anti-Nkx2.5 antibody [EPR20168] (ab205263) at 1/1000 dilution
Lane 1 : Human fetal heart muscle tissue lysate Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution