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RecombinantRabMAb

Recombinant Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)

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Western blot - Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)
  • Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20168] to Nkx2.5 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

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Protein
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Recombinant Human Nkx2.5 protein (ab152305)

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Overview

  • Product name

    Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free
    See all Nkx2.5 primary antibodies
  • Description

    Rabbit monoclonal [EPR20168] to Nkx2.5 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Nkx2.5. The exact sequence is proprietary.
    Database link: P52952

  • Positive control

    • WB: Human fetal heart muscle tissue lysate; HEK-293T and HeLa whole cell lysate.
  • General notes

    Ab228335 is the carrier-free version of ab205263. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab228335 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20168
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Cardiomyocytes

Associated products

  • Alternative Versions

    • Anti-Nkx2.5 antibody [EPR20168] (ab205263)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Nkx2.5 protein (ab152305)
  • Related Products

    • Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Applications

Our Abpromise guarantee covers the use of ab228335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 30-42 kDa (predicted molecular weight: 35 kDa).

Target

  • Function

    Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
  • Tissue specificity

    Expressed only in the heart.
  • Involvement in disease

    Atrial septal defect 7, with or without atrioventricular conduction defects
    Tetralogy of Fallot
    Conotruncal heart malformations
    Hypothyroidism, congenital, non-goitrous, 5
    Ventricular septal defect 3
    Hypoplastic left heart syndrome 2
    Asplenia, isolated congenital
  • Sequence similarities

    Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P52952 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1482 Human
    • Omim: 600584 Human
    • SwissProt: P52952 Human
    • Unigene: 54473 Human
    • Alternative names

      • NKX2.5, mouse, homolog of antibody
      • Cardiac-specific homeobox 1 antibody
      • Cardiac-specific homeobox antibody
      • CHNG5 antibody
      • CSX antibody
      • CSX1 antibody
      • FLJ52202 antibody
      • FLJ97166 antibody
      • FLJ97195 antibody
      • FLJ97197 antibody
      • FLJ99536 antibody
      • HLHS2 antibody
      • Homeobox protein CSX antibody
      • Homeobox protein NK-2 homolog E antibody
      • Homeobox protein Nkx 2.5 antibody
      • Homeobox protein Nkx-2.5 antibody
      • NK2 homeobox 5 antibody
      • NK2 transcription factor related locus 5 antibody
      • NK2 transcription factor related, locus 5 (Drosophila) antibody
      • NK2, Drosophila, homolog of, E antibody
      • NKX2-5 antibody
      • NKX2.5 antibody
      • NKX25_HUMAN antibody
      • Nkx2E antibody
      • NKX4-1 antibody
      • Tinman paralog antibody
      • VSD3 antibody
      see all

    Images

    • Western blot - Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)
      Western blot - Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)
      All lanes : Anti-Nkx2.5 antibody [EPR20168] (ab205263) at 1/1000 dilution

      Lane 1 : Human fetal heart muscle tissue lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 35 kDa
      Observed band size: 30-42 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution: 5% NFDM/TBST

      The molecular masses observed are consistent with what has been described in the literature (PMID: 21447557)

      Negative control: MCF7 (PMID: 15340050).

      The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205263).

    • Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)
      Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab228335? Please let us know so that we can cite the reference in this datasheet.

    ab228335 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab228335.
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