Recombinant Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)
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- References
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Overview
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Product name
Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free
See all Nkx2.5 primary antibodies -
Description
Rabbit monoclonal [EPR20168] to Nkx2.5 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Nkx2.5. The exact sequence is proprietary.
Database link: P52952 -
Positive control
- WB: Human fetal heart muscle tissue lysate; HEK-293T and HeLa whole cell lysate.
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General notes
Ab228335 is the carrier-free version of ab205263. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab228335 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20168 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab228335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 30-42 kDa (predicted molecular weight: 35 kDa). |
Target
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Function
Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development. -
Tissue specificity
Expressed only in the heart. -
Involvement in disease
Atrial septal defect 7, with or without atrioventricular conduction defects
Tetralogy of Fallot
Conotruncal heart malformations
Hypothyroidism, congenital, non-goitrous, 5
Ventricular septal defect 3
Hypoplastic left heart syndrome 2
Asplenia, isolated congenital -
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1482 Human
- Omim: 600584 Human
- SwissProt: P52952 Human
- Unigene: 54473 Human
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Alternative names
- NKX2.5, mouse, homolog of antibody
- Cardiac-specific homeobox 1 antibody
- Cardiac-specific homeobox antibody
see all
Images
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![Western blot - Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)](https://www.abcam.com/ps/products/228/ab228335/Images/ab228335-291686-anti-nkx25-antibody-epr20168-western-blot.jpg)
Western blot - Anti-Nkx2.5 antibody [EPR20168] - BSA and Azide free (ab228335)All lanes : Anti-Nkx2.5 antibody [EPR20168] (ab205263) at 1/1000 dilution
Lane 1 : Human fetal heart muscle tissue lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 30-42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution: 5% NFDM/TBST
The molecular masses observed are consistent with what has been described in the literature (PMID: 21447557)
Negative control: MCF7 (PMID: 15340050).
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205263).
Datasheets and documents
References
ab228335 has not yet been referenced specifically in any publications.
