Overview

  • Product name

    Anti-NKX2.8 antibody
  • Description

    Rabbit polyclonal to NKX2.8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to 19 internal sequence amino acids of Human NKX2.8 (NP_055175).

  • Positive control

    • Rat Liver Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab125040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 26 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

Images

  • Immunohistochemistry of NKX2.8 in Human liver tissue with ab125040 at 2.5 μg/mL.

  • Immunofluorescence of NKX2.8 in Human liver tissue with ab125040 at 20 ug/mL.
  • All lanes : Anti-NKX2.8 antibody (ab125040) at 1 µg/ml

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat liver tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 26 kDa

References

This product has been referenced in:

  • Zhu J  et al. NKX2-8 deletion-induced reprogramming of fatty acid metabolism confers chemoresistance in epithelial ovarian cancer. EBioMedicine 43:238-252 (2019). Read more (PubMed: 31047858) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton 0.5%
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Sueli Marques

Verified customer

Submitted Dec 22 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up