Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14970] to Nkx3.1
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-Nkx3.1 antibody [EPR14970]
See all Nkx3.1 primary antibodies
DescriptionRabbit monoclonal [EPR14970] to Nkx3.1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Nkx3.1 aa 50-200. The exact sequence is proprietary.
Database link: Q99801
- human prostate tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab186413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.|
|IHC-P||1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionTranscription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Tissue specificityHighly expressed in the prostate and, at a lower level, in the testis.
Sequence similaritiesBelongs to the NK-3 homeobox family.
Contains 1 homeobox DNA-binding domain.
modificationsUbiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues.
- Information by UniProt
- BAPX 2 antibody
- BAPX2 antibody
- Homeobox protein NK-3 homolog A antibody
Immunohistochemical analysis of paraffin embedded human prostate tissue labeling Nkx3.1 with ab186413 at 1/4000 dilution and ready to use HRP Polymer to Rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Lane 1 : Anti-Nkx3.1 antibody [EPR14970] (ab186413) at 0.988 µg/ml (Exposure time: 10 seconds)
Lane 2 : Anti-Nkx3.1 antibody [EPR14970] (ab186413) at 0.988 µg/ml (Exposure time: 3 minutes)
Lane 1 : LNCaP cell lysate
Lane 2 : PC3 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer used was 5% NFDM/TBST.
ab186413 has not yet been referenced specifically in any publications.