Overview

  • Product name

    Anti-Nkx3.1 antibody [EPR14970]
    See all Nkx3.1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14970] to Nkx3.1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Nkx3.1 aa 50-200. The exact sequence is proprietary.
    Database link: Q99801

  • Positive control

    • human prostate tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab186413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
IHC-P 1/4000.

Target

  • Function

    Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
  • Tissue specificity

    Highly expressed in the prostate and, at a lower level, in the testis.
  • Sequence similarities

    Belongs to the NK-3 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications

    Ubiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BAPX 2 antibody
    • BAPX2 antibody
    • Homeobox protein NK-3 homolog A antibody
    • Homeobox protein Nkx 3.1 antibody
    • Homeobox protein Nkx-3.1 antibody
    • Homeobox protein Nkx3.1 antibody
    • NK homeobox (Drosophila) family 3 A antibody
    • NK homeobox family 3 A antibody
    • NK homeobox, family 3, member A antibody
    • NK3 homeobox 1 antibody
    • NK3 transcription factor homolog A antibody
    • NK3 transcription factor related locus 1 antibody
    • NKX 3 antibody
    • Nkx 3.1 antibody
    • NKX 3A antibody
    • NKX3 1 antibody
    • NKX3 antibody
    • Nkx3-1 antibody
    • NKX3.1 antibody
    • Nkx3.1, mouse, homolog of antibody
    • NKX31_HUMAN antibody
    • NKX3A antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded human prostate tissue labeling Nkx3.1 with ab186413 at 1/4000 dilution and ready to use HRP Polymer to Rabbit IgG.  Counterstained with Hematoxylin.

  • Lane 1 : Anti-Nkx3.1 antibody [EPR14970] (ab186413) at 0.988 µg/ml (Exposure time: 10 seconds)
    Lane 2 : Anti-Nkx3.1 antibody [EPR14970] (ab186413) at 0.988 µg/ml (Exposure time: 3 minutes)

    Lane 1 : LNCaP cell lysate
    Lane 2 : PC3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 26 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer used was 5% NFDM/TBST.

References

ab186413 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (bladder)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6.0, pH2.0 or Tris-EDTA pH9.0
Permeabilization
No
Specification
bladder
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 27 2018

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