Recombinant Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
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Overview
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Product name
Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free
See all nkx6.1 primary antibodies -
Description
Rabbit monoclonal [EPR20328] to nkx6.1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human nkx6.1 aa 200-300. The exact sequence is proprietary.
Database link: P78426 -
General notes
Ab251565 is the carrier-free version of ab221544. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab251565 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR20328 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Related Products
Applications
Our Abpromise guarantee covers the use of ab251565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 38 kDa. Detection of endogenous expression of nkx6.1 in WB will require optimization. Under our experimental conditions we only detected weak endogenous expression in mouse lysates.
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Target
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Function
Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals. -
Tissue specificity
Pancreatic beta cells. -
Sequence similarities
Contains 1 homeobox DNA-binding domain. -
Domain
The C-terminal domain contributes to sequence-specific DNA-binding. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4825 Human
- Entrez Gene: 18096 Mouse
- Entrez Gene: 65193 Rat
- Omim: 602563 Human
- SwissProt: P78426 Human
- SwissProt: Q99MA9 Mouse
- SwissProt: O35762 Rat
- Unigene: 546270 Human
see all -
Alternative names
- Homeobox protein NK-6 homolog A antibody
- Homeobox protein Nkx-6.1 antibody
- NK homeo box, family 6, member A antibody
see all
Datasheets and documents
References
ab251565 has not yet been referenced specifically in any publications.
