RecombinantRabMAb

Recombinant Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
  • Western blot - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
  • Western blot - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
  • Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20328] to nkx6.1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free
    See all nkx6.1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20328] to nkx6.1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human nkx6.1 aa 200-300. The exact sequence is proprietary.
    Database link: P78426

  • General notes

    Ab251565 is the carrier-free version of ab221544. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251565 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab251565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.

Detection of endogenous expression of nkx6.1 in WB will require optimization. Under our experimental conditions we only detected weak endogenous expression in mouse lysates.

Target

  • Function

    Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals.

  • Tissue specificity

    Pancreatic beta cells.

  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.

  • Domain

    The C-terminal domain contributes to sequence-specific DNA-binding.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Homeobox protein NK-6 homolog A antibody
    • Homeobox protein Nkx-6.1 antibody
    • NK homeo box, family 6, member A antibody
    • NK homeobox (Drosophila), family 6, A antibody
    • NK homeobox, family 6, A antibody
    • NK6 homeobox 1 antibody
    • NK6 transcription factor homolog A antibody
    • NK6 transcription factor related, locus 1 antibody
    • NK6 transcription factor related, locus 1 (Drosophila) antibody
    • NKX6-1 antibody
    • NKX6.1 antibody
    • NKX61 antibody
    • NKX61_HUMAN antibody
    • NKX6A antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)

    This data was developed using ab221544, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of human pancreas (PMID: 8702531). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)

    This data was developed using ab221544, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human pancreatic neuroendocrine neoplasm tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Mainly nuclear staining on tumor cells of human pancreatic neuroendocrine neoplasm (PMID: 25871618). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    This data was developed using ab221544, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of mouse pancreas (PMID: 8702531). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    This data was developed using ab221544, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on rat pancreas (PMID: 8702531). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    This data was developed using ab221544, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Negative control: no staining on human colon (PMID: 25871618). Counter stained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Western blot - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Anti-nkx6.1 antibody [EPR20328] (ab221544) at 1/1000 dilution + Human nkx6.1 recombinant protein (aa (1-48) & aa(66-120) & aa(179-240) with a His-tag) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    This data was developed using ab221544, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human nkx6.1 recombinant protein contains aa1-48, aa66-120, and aa179-240 with a His-tag.

  • Western blot - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Western blot - Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Anti-nkx6.1 antibody [EPR20328] (ab221544) at 1/200 dilution + Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa


    Exposure time: 3 minutes


    This data was developed using ab221544, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
    Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab251565? Please let us know so that we can cite the reference in this datasheet.

ab251565 has not yet been referenced specifically in any publications.

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