Recombinant
RabMAb

Recombinant Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)

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Overview

  • Product name

    Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free
    See all nkx6.1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20405] to nkx6.1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment within Human nkx6.1 aa 1-250. The exact sequence is proprietary.
    Database link: P78426

  • General notes

    Ab240398 is the carrier-free version of ab221549. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240398 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Under our testing conditions we used a high sensitivity exposure system to obtain this result. Customers are recommended to optimize WB testing conditions.

 
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals.

  • Tissue specificity

    Pancreatic beta cells.

  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.

  • Domain

    The C-terminal domain contributes to sequence-specific DNA-binding.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Homeobox protein NK-6 homolog A antibody
      • Homeobox protein Nkx-6.1 antibody
      • NK homeo box, family 6, member A antibody
      • NK homeobox (Drosophila), family 6, A antibody
      • NK homeobox, family 6, A antibody
      • NK6 homeobox 1 antibody
      • NK6 transcription factor homolog A antibody
      • NK6 transcription factor related, locus 1 antibody
      • NK6 transcription factor related, locus 1 (Drosophila) antibody
      • NKX6-1 antibody
      • NKX6.1 antibody
      • NKX61 antibody
      • NKX61_HUMAN antibody
      • NKX6A antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of human pancreas (PMID: 8702531). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221549).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)

      Immunohistochemical analysis of paraffin-embedded human pancreatic neuroendocrine neoplasm tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Mainly nuclear staining on tumor cells of human pancreatic neuroendocrine neoplasm (PMID: 25871618). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221549).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)

      Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of mouse pancreas (PMID: 8702531). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221549).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Negative control: no staining on human colon (PMID: 25871618).

      Counter stained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221549).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)
      Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling nkx6.1 with ab221549  at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on islet of mouse pancreas is observed.

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221549).

    References

    ab240398 has not yet been referenced specifically in any publications.

    Publishing research using ab240398? Please let us know so that we can cite the reference in this datasheet.

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