Overview

  • Product name

    Anti-nkx6.3 antibody
  • Description

    Rabbit polyclonal to nkx6.3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human nkx6.3 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab107907)

  • Positive control

    • This antibody gave a positive signal in the following lysates: E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab93235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 mg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

Target

Images

  • Anti-nkx6.3 antibody (ab93235) at 1 µg/ml + E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa
    Observed band size: 28 kDa
    Additional bands at: 38 kDa, 42 kDa, 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes
  • ICC/IF image of ab93235 stained SK-N-SH cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93235, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab93235 has not yet been referenced specifically in any publications.

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