Product nameAnti-nkx6.3 antibody
DescriptionRabbit polyclonal to nkx6.3
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human nkx6.3 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following lysates: E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 mg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
FunctionPutative transcription factor, which may be involved in patterning of central nervous system and pancreas.
Sequence similaritiesContains 1 homeobox DNA-binding domain.
- Information by UniProt
- FLJ25169 antibody
- Homeobox protein Nkx 6.3 antibody
- Homeobox protein Nkx-6.3 antibody
Anti-nkx6.3 antibody (ab93235) at 1 µg/ml + E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate at 10 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Additional bands at: 38 kDa, 42 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
ICC/IF image of ab93235 stained SK-N-SH cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93235, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab93235 has not yet been referenced specifically in any publications.