Anti-NLE1/nle antibody (ab227554)
Key features and details
- Rabbit polyclonal to NLE1/nle
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NLE1/nle antibody
See all NLE1/nle primary antibodies -
Description
Rabbit polyclonal to NLE1/nle -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Chimpanzee, Rhesus monkey -
Immunogen
Recombinant fragment within Human NLE1/nle (internal sequence). The exact sequence is proprietary.
Database link: Q9NVX2 -
Positive control
- WB: HEK-293T whole cell and nuclear extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
Target
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Sequence similarities
Belongs to the NLE1/RSA4 family.
Contains 8 WD repeats. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 509793 Cow
- Entrez Gene: 54475 Human
- Entrez Gene: 217011 Mouse
- Entrez Gene: 303372 Rat
- SwissProt: Q58D20 Cow
- SwissProt: Q9NVX2 Human
- SwissProt: Q8VEJ4 Mouse
- Unigene: 85570 Human
see all -
Alternative names
- AL022765 antibody
- BC018399 antibody
- FLJ10458 antibody
see all
Images
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All lanes : Anti-NLE1/nle antibody (ab227554) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa10% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227554 has not yet been referenced specifically in any publications.