Key features and details
- Rabbit polyclonal to NLE1/nle
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NLE1/nle antibody
See all NLE1/nle primary antibodies
DescriptionRabbit polyclonal to NLE1/nle
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Orangutan
Recombinant fragment corresponding to Human NLE1/nle aa 206-485.
SWEPLHANPECRYVASSSKDGSVRIWDTTAGRCERILTGHTQSVTCLRWG GDGLLYSASQDRTIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGA FEPAEASVNPQDLQGSLQELKERALSRYNLVRGQGPERLVSGSDDFTLFL WSPAEDKKPLTRMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGK YLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVWDVKAQKLAMDLPGH ADEVYAVDWSPDGQRVASGGKDKCLRIWRR
Database link: Q9NVX2
- WB: PC-3 whole cell lysate. IHC-P: Human gastric cancer and small intestine tissues.
This product was previously labelled as NLE1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
Sequence similaritiesBelongs to the NLE1/RSA4 family.
Contains 8 WD repeats.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- AL022765 antibody
- BC018399 antibody
- FLJ10458 antibody
Anti-NLE1/nle antibody (ab230212) at 1/1000 dilution + PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Paraffin-embedded human small intestine tissue stained for NLE1/nle using ab230212 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human gastric cancer tissue stained for NLE1/nle using ab230212 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230212 has not yet been referenced specifically in any publications.