• Product name

  • Description

    Rabbit polyclonal to NLK
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Dog, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human NLK.

    Read Abcam's proprietary immunogen policy (Peptide available as ab26986.)

  • Positive control

    • This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Mouse Lung Normal; Formalin Fixed Paraffin Embedded Mouse Brain Normal. This antibody gave a positive signal in the following cell types: U-87 MG.



Our Abpromise guarantee covers the use of ab26050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.


  • Function

    Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational

    Dually phosphorylated on Thr-303 and Tyr-305, which activates the enzyme.
  • Cellular localization

    Nucleus. Cytoplasm. Predominantly nuclear. A smaller fraction is cytoplasmic.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp761G1211 antibody
    • FLJ21033 antibody
    • LAK1 antibody
    • Nemo like kinase antibody
    • Nemo-like kinase antibody
    • Nlk antibody
    • NLK_HUMAN antibody
    • Protein LAK1 antibody
    • Serine/threonine protein kinase NLK antibody
    • Serine/threonine-protein kinase NLK antibody
    see all


  • Anti-NLK was used in standard Vector ABC protocol on paraffin embedded mouse lung with the following modifications: HIER with Vector antigen unmasking solution for 25 minutes at 98C. Used a 1:100 dilution in PBS/Triton-X 4C overnight.
  • Adult mouse brain fixed in 10% neutral buffered formulin. Antigen retrieval was performed using Tris-EDTA pH 9.0. The tissue was incubated with ab26050 at a concentration of 2.5µg/ml for 16 hours at 4°C. Nuclear staining can be clearly seen. 

  • ICC/IF image of ab26050 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab26050 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Song L  et al. c-Kit modifies the inflammatory status of smooth muscle cells. PeerJ 5:e3418 (2017). WB ; Mouse . Read more (PubMed: 28626608) »
  • Rydell-Törmänen K  et al. Aberrant nonfibrotic parenchyma in idiopathic pulmonary fibrosis is correlated with decreased ß-catenin inhibition and increased Wnt5a/b interaction. Physiol Rep 4:N/A (2016). IHC-P ; Human . Read more (PubMed: 26997628) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (Kidney cancer cell)
Loading amount
20 µg
Kidney cancer cell
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 16 2010

Western blot
Human Cell lysate - whole cell (Human prostate cancer)
Loading amount
50 µg
Human prostate cancer
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%

Mrs. Tiffany Pitts

Verified customer

Submitted May 31 2007

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