Overview

  • Product name

    Anti-NLP1 antibody [EPR16545]
    See all NLP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16545] to NLP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NLP1 aa 1-150. The exact sequence is proprietary.
    Database link: O15504

  • Positive control

    • 293, K562, HeLa, PC12, NIH 3T3, C6, RAW 264.7 cell lysates, Human fetal liver, Mouse brain, heart, kidney and spleen, Rat heart and brain lysates; Human kidney clear cell carcinoma, tonsil and Mouse lung tissues; HeLa cells.
  • General notes

     

     

    Previously labelled as NUPL2.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.
IP 1/40.

Target

  • Relevance

    CG1 is required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope.
  • Cellular localization

    Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Note: Excluded from the nucleolus.
  • Database links

  • Alternative names

    • CG1 antibody
    • NLP1 antibody
    • Nucleoporin hCG1 antibody
    • Nucleoporin like protein 2 antibody
    • NUP42 homolog antibody
    • NUPL2 antibody
    see all

Images

  • Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NLP1 with ab192609 at 1/100 dilution (6.3μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue). Negative control also shown.

  • Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling NLP1 with ab192609 at 1/100 dilution (6 μg/ml); followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

  • Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling NLP1 with ab192609 at 1/100 dilution (6 μg/ml); followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling NLP1 with ab192609 at 1/100 dilution (6 μg/ml);  followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

  • All lanes : Anti-NLP1 antibody [EPR16545] (ab192609) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate
    Lane 5 : Mouse brain lysate
    Lane 6 : Mouse heart lysate
    Lane 7 : Mouse kidney lysate
    Lane 8 : Mouse spleen lysate
    Lane 9 : Rat brain lysate
    Lane 10 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa

  • Anti-NLP1 antibody [EPR16545] (ab192609) at 1/1000 dilution + Human fetal liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa

  • All lanes : Anti-NLP1 antibody [EPR16545] (ab192609) at 1/10000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa

  • Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab192609 at 1/40 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab192609 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Tissue lysate - whole (Human cerebellum lysate)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Human cerebellum lysate
Blocking step
Odyssey Blocker TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 21 2017

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