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Rabbit polyclonal to NLRC3
HumanPredicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Human NLRC3.
(Peptide available as ab90252.)
- This antibody gave a positive signal in human lymph node tissue lysate and the following whole cell lysates: Raji; Ramos; Jurkat.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa (predicted molecular weight: 114 kDa).
May modulate T-cell activation. Decreases the transcription of genes that are normally up-regulated after T-cell stimulation. Delays degradation of NFKBIA/IKBA.
Detected in peripheral blood mononuclear cells.
Belongs to the NLRP family.
Contains 16 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.
Information by UniProt
- CARD15-like antibody
- CARD15-like protein antibody
- Caterpiller 16.2 antibody
Western blot - Anti-NLRC3 antibody (ab77817)
All lanes :
Anti-NLRC3 antibody (ab77817) at 1 µg/mlLane 1 :
Human lymph node tissue lysate - total protein (ab29871
)Lane 2 :
Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 3 :
Ramos (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 4 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
114 kDaObserved band size:
105 kDa (why is the actual band size different from the predicted?
)Additional bands at:
33 kDa, 70 kDa. We are unsure as to the identity of these extra bands.Exposure time:
NLRC3 has four possible isoforms which have the following predicted molecular weights, 114, 119, 83 and 111 kDa (SwissProt data).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"