Overview

  • Product name

  • Description

    Rabbit polyclonal to NLRC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Pig
  • Immunogen

    Synthetic peptide corresponding to Human NLRC3 aa 1050 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90252)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: Raji; Ramos; Jurkat.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa (predicted molecular weight: 114 kDa).

Target

  • Function

    May modulate T-cell activation. Decreases the transcription of genes that are normally up-regulated after T-cell stimulation. Delays degradation of NFKBIA/IKBA.
  • Tissue specificity

    Detected in peripheral blood mononuclear cells.
  • Sequence similarities

    Belongs to the NLRP family.
    Contains 16 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CARD15-like antibody
    • CARD15-like protein antibody
    • Caterpiller 16.2 antibody
    • Caterpiller protein 16.2 antibody
    • CLR16.2 antibody
    • FLJ00348 antibody
    • NLR family CARD domain containing 3 antibody
    • Nlrc3 antibody
    • NLRC3_HUMAN antibody
    • NOD-like receptor C3 antibody
    • NOD3 antibody
    • NOD3 protein antibody
    • Nucleotide-binding oligomerization domain protein 3 antibody
    • Nucleotide-binding oligomerization domain protein 3 caterpiller 16.2 antibody
    • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3 antibody
    • Protein NLRC3 antibody
    see all

Images

  • All lanes : ab77817 at 1 µg/ml

    Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 114 kDa
    Observed band size: 105 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 33 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 minutes


    NLRC3 has four possible isoforms which have the following predicted molecular weights, 114, 119, 83 and 111 kDa (SwissProt data).

References

This product has been referenced in:

  • Eren E  et al. NLRC3 protein inhibits inflammation by disrupting NALP3 inflammasome assembly via competition with the adaptor protein ASC for pro-caspase-1 binding. J Biol Chem 292:12691-12701 (2017). Read more (PubMed: 28584053) »
  • Guo L  et al. NLRC3 promotes host resistance against Pseudomonas aeruginosa-induced keratitis by promoting the degradation of IRAK1. Int J Mol Med 40:898-906 (2017). Read more (PubMed: 28731142) »
See all 3 Publications for this product

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