Anti-NLRC3 antibody (ab77817)
Key features and details
- Rabbit polyclonal to NLRC3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-NLRC3 antibody -
Description
Rabbit polyclonal to NLRC3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Pig -
Immunogen
Synthetic peptide corresponding to Human NLRC3 aa 1050 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab90252) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: Raji; Ramos; Jurkat.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa (predicted molecular weight: 114 kDa).
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa (predicted molecular weight: 114 kDa). |
Target
-
Function
May modulate T-cell activation. Decreases the transcription of genes that are normally up-regulated after T-cell stimulation. Delays degradation of NFKBIA/IKBA. -
Tissue specificity
Detected in peripheral blood mononuclear cells. -
Sequence similarities
Belongs to the NLRP family.
Contains 16 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 197358 Human
- Entrez Gene: 268857 Mouse
- SwissProt: Q7RTR2 Human
- SwissProt: Q5DU56 Mouse
- Unigene: 592091 Human
- Unigene: 741496 Human
- Unigene: 215994 Mouse
-
Alternative names
- CARD15-like antibody
- CARD15-like protein antibody
- Caterpiller 16.2 antibody
see all
Images
-
All lanes : ab77817 at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutesNLRC3 has four possible isoforms which have the following predicted molecular weights, 114, 119, 83 and 111 kDa (SwissProt data).
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab77817 has been referenced in 4 publications.
- Wang P et al. LDL-induced NLRC3 inflammasome activation in cardiac fibroblasts contributes to cardiomyocytic dysfunction. Mol Med Rep 24:N/A (2021). PubMed: 34036387
- Eren E et al. NLRC3 protein inhibits inflammation by disrupting NALP3 inflammasome assembly via competition with the adaptor protein ASC for pro-caspase-1 binding. J Biol Chem 292:12691-12701 (2017). PubMed: 28584053
- Guo L et al. NLRC3 promotes host resistance against Pseudomonas aeruginosa-induced keratitis by promoting the degradation of IRAK1. Int J Mol Med 40:898-906 (2017). PubMed: 28731142
- Gültekin Y et al. Overexpressed NLRC3 acts as an anti-inflammatory cytosolic protein. J Innate Immun 7:25-36 (2015). PubMed: 25277106