Anti-NLRC4 antibody (ab99860)

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Western blot - Anti-NLRC4 antibody (ab99860)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC4 antibody (ab99860)

Key features and details

  • Rabbit polyclonal to NLRC4
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-NLRC4 antibody
    See all NLRC4 primary antibodies

  • Description

    Rabbit polyclonal to NLRC4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment corresponding to Human NLRC4 aa 764-1019. (BC031555)

  • Positive control

    • Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282) can be used as a positive control in WB. Human fetal brain lysate

  • General notes

     This product was previously labelled as CARD12

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab99860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 116 kDa.
IHC-P
1/100 - 1/500.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 116 kDa.
IHC-P
1/100 - 1/500.

Target

  • Function

    Plays a role in the promotion of apoptosis.

  • Tissue specificity

    Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.

  • Sequence similarities

    Contains 1 CARD domain.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.

  • Cellular localization

    Cytoplasm. Cytoplasmic filaments.
  • Information by UniProt

  • Database links

    • Alternative names

      • and NACHT-containing protein antibody
      • CARD 12 antibody
      • CARD antibody
      • CARD LRR and NACHT containing protein 1 antibody
      • CARD LRR and NACHT containing protein antibody
      • CARD12 antibody
      • Caspase recruitment domain containing protein 12 antibody
      • Caspase recruitment domain family member 12 antibody
      • Caspase recruitment domain-containing protein 12 antibody
      • CLAN 1 antibody
      • CLAN A antibody
      • CLAN antibody
      • CLAN B antibody
      • CLAN C antibody
      • CLAN D antibody
      • Clan protein antibody
      • CLAN1 antibody
      • CLANA antibody
      • CLANB antibody
      • CLANC antibody
      • CLAND antibody
      • CLR 2.1 antibody
      • CLR2.1 antibody
      • ICE protease activating factor antibody
      • Ice protease-activating factor antibody
      • Ipaf antibody
      • LRR antibody
      • NLR family CARD domain containing 4 antibody
      • NLR family CARD domain-containing protein 4 antibody
      • NLRC 4 antibody
      • NLRC4 antibody
      • NLRC4_HUMAN antibody
      • NOD like receptor C4 antibody
      • Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 4 antibody
      • UNQ6189/PRO20215 antibody
      see all

    Images

    • Western blot - Anti-NLRC4 antibody (ab99860)
      Western blot - Anti-NLRC4 antibody (ab99860)
      Anti-NLRC4 antibody (ab99860) at 1/500 dilution + Human fetal brain lysate

      Predicted band size: 116 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC4 antibody (ab99860)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC4 antibody (ab99860)
      Immunohistochemical staining of formalin-fixed paraffin-embedded human fetal lung showing cytoplasmic staining with ab99860 at a dilution of 1/100.

    References (3)

    Publishing research using ab99860? Please let us know so that we can cite the reference in this datasheet.

    ab99860 has been referenced in 3 publications.

    • Jin H & Kim HJ NLRC4, ASC and Caspase-1 Are Inflammasome Components that Are Mediated by P2Y2R Activation in Breast Cancer Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32397236
    • Lin ZH  et al. Methylene Blue Mitigates Acute Neuroinflammation after Spinal Cord Injury through Inhibiting NLRP3 Inflammasome Activation in Microglia. Front Cell Neurosci 11:391 (2017). PubMed: 29311826
    • Ataide MA  et al. Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection. PLoS Pathog 10:e1003885 (2014). PubMed: 24453977

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