Anti-NLRC4 antibody (ab99860)
Key features and details
- Rabbit polyclonal to NLRC4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NLRC4 antibody
See all NLRC4 primary antibodies -
Description
Rabbit polyclonal to NLRC4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human NLRC4 aa 764-1019. (BC031555)
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Positive control
- Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282) can be used as a positive control in WB. Human fetal brain lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200ul sterile distilled water -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 116 kDa.
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| IHC-P |
1/100 - 1/500.
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| Notes |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 116 kDa. |
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IHC-P
1/100 - 1/500. |
Target
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Function
Plays a role in the promotion of apoptosis. -
Tissue specificity
Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain. -
Sequence similarities
Contains 1 CARD domain.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Cytoplasm. Cytoplasmic filaments. - Information by UniProt
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Database links
- Entrez Gene: 58484 Human
- Entrez Gene: 268973 Mouse
- Entrez Gene: 298784 Rat
- Omim: 606831 Human
- SwissProt: Q9NPP4 Human
- SwissProt: Q3UP24 Mouse
- SwissProt: F1M649 Rat
- Unigene: 574741 Human
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Alternative names
- and NACHT-containing protein antibody
- CARD 12 antibody
- CARD antibody
see all
Images
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Western blot - Anti-NLRC4 antibody (ab99860)Anti-NLRC4 antibody (ab99860) at 1/500 dilution + Human fetal brain lysate
Predicted band size: 116 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC4 antibody (ab99860)Immunohistochemical staining of formalin-fixed paraffin-embedded human fetal lung showing cytoplasmic staining with ab99860 at a dilution of 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab99860 has been referenced in 5 publications.
- Jin H & Kim HJ NLRC4, ASC and Caspase-1 Are Inflammasome Components that Are Mediated by P2Y2R Activation in Breast Cancer Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32397236
- Liu G et al. USP7 Inhibition Alleviates H2O2-Induced Injury in Chondrocytes via Inhibiting NOX4/NLRP3 Pathway. Front Pharmacol 11:617270 (2020). PubMed: 33584299
- Zhang P et al. Hyperglycemia-induced inflamm-aging accelerates gingival senescence via NLRC4 phosphorylation. J Biol Chem 294:18807-18819 (2019). PubMed: 31676687
- Lin ZH et al. Methylene Blue Mitigates Acute Neuroinflammation after Spinal Cord Injury through Inhibiting NLRP3 Inflammasome Activation in Microglia. Front Cell Neurosci 11:391 (2017). PubMed: 29311826
- Ataide MA et al. Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection. PLoS Pathog 10:e1003885 (2014). PubMed: 24453977