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  1. Link

    nlrc4-antibody-ab99860.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-NLRC4 antibody (ab99860)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (5)

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Western blot - Anti-NLRC4 antibody (ab99860)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC4 antibody (ab99860)

Key features and details

  • Rabbit polyclonal to NLRC4
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Recombinant Human NLRC4 protein (ab127060)
Primary
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Anti-NLRC4 antibody [EPR19733] (ab201792)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-NLRC4 antibody
    See all NLRC4 primary antibodies
  • Description

    Rabbit polyclonal to NLRC4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human NLRC4 aa 764-1019. (BC031555)

  • Positive control

    • Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282) can be used as a positive control in WB. Human fetal brain lysate
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Lyophilized:Reconstitute with 200ul sterile distilled water
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • CARD Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human NLRC4 protein (ab127060)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab99860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/1000. Predicted molecular weight: 116 kDa.
IHC-P
1/100 - 1/500.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 116 kDa.
IHC-P
1/100 - 1/500.

Target

  • Function

    Plays a role in the promotion of apoptosis.
  • Tissue specificity

    Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.
  • Sequence similarities

    Contains 1 CARD domain.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Cellular localization

    Cytoplasm. Cytoplasmic filaments.
  • Target information above from: UniProt accession Q9NPP4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 58484 Human
    • Entrez Gene: 268973 Mouse
    • Entrez Gene: 298784 Rat
    • Omim: 606831 Human
    • SwissProt: Q9NPP4 Human
    • SwissProt: Q3UP24 Mouse
    • SwissProt: F1M649 Rat
    • Unigene: 574741 Human
    • Alternative names

      • and NACHT-containing protein antibody
      • CARD 12 antibody
      • CARD antibody
      • CARD LRR and NACHT containing protein 1 antibody
      • CARD LRR and NACHT containing protein antibody
      • CARD12 antibody
      • Caspase recruitment domain containing protein 12 antibody
      • Caspase recruitment domain family member 12 antibody
      • Caspase recruitment domain-containing protein 12 antibody
      • CLAN 1 antibody
      • CLAN A antibody
      • CLAN antibody
      • CLAN B antibody
      • CLAN C antibody
      • CLAN D antibody
      • Clan protein antibody
      • CLAN1 antibody
      • CLANA antibody
      • CLANB antibody
      • CLANC antibody
      • CLAND antibody
      • CLR 2.1 antibody
      • CLR2.1 antibody
      • ICE protease activating factor antibody
      • Ice protease-activating factor antibody
      • Ipaf antibody
      • LRR antibody
      • NLR family CARD domain containing 4 antibody
      • NLR family CARD domain-containing protein 4 antibody
      • NLRC 4 antibody
      • NLRC4 antibody
      • NLRC4_HUMAN antibody
      • NOD like receptor C4 antibody
      • Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 4 antibody
      • UNQ6189/PRO20215 antibody
      see all

    Images

    • Western blot - Anti-NLRC4 antibody (ab99860)
      Western blot - Anti-NLRC4 antibody (ab99860)
      Anti-NLRC4 antibody (ab99860) at 1/500 dilution + Human fetal brain lysate

      Predicted band size: 116 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC4 antibody (ab99860)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC4 antibody (ab99860)
      Immunohistochemical staining of formalin-fixed paraffin-embedded human fetal lung showing cytoplasmic staining with ab99860 at a dilution of 1/100.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab99860? Please let us know so that we can cite the reference in this datasheet.

    ab99860 has been referenced in 5 publications.

    • Jin H & Kim HJ NLRC4, ASC and Caspase-1 Are Inflammasome Components that Are Mediated by P2Y2R Activation in Breast Cancer Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32397236
    • Liu G  et al. USP7 Inhibition Alleviates H2O2-Induced Injury in Chondrocytes via Inhibiting NOX4/NLRP3 Pathway. Front Pharmacol 11:617270 (2020). PubMed: 33584299
    • Zhang P  et al. Hyperglycemia-induced inflamm-aging accelerates gingival senescence via NLRC4 phosphorylation. J Biol Chem 294:18807-18819 (2019). PubMed: 31676687
    • Lin ZH  et al. Methylene Blue Mitigates Acute Neuroinflammation after Spinal Cord Injury through Inhibiting NLRP3 Inflammasome Activation in Microglia. Front Cell Neurosci 11:391 (2017). PubMed: 29311826
    • Ataide MA  et al. Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection. PLoS Pathog 10:e1003885 (2014). PubMed: 24453977

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-NLRC4 antibody

    Poor
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (vascular smooth muscle cells)
    Gel Running Conditions
    Non-reduced Denaturing (4-15%)
    Loading amount
    25 µg
    Specification
    vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 24 2021

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