Anti-NLRC4 antibody (ab99860)
- Datasheet
- References (2)
- Protocols
Overview
-
Product name
Anti-NLRC4 antibody
See all NLRC4 primary antibodies -
Description
Rabbit polyclonal to NLRC4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Recombinant fragment corresponding to Human NLRC4 aa 764-1019. (BC031555)
-
Positive control
- Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282) can be used as a positive control in WB. Human fetal brain lysate
-
General notes
This product was previously labelled as CARD12
Properties
-
Form
Lyophilised:Reconstitute with 200ul sterile distilled water -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab99860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 116 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
-
Function
Plays a role in the promotion of apoptosis. -
Tissue specificity
Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain. -
Sequence similarities
Contains 1 CARD domain.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Cytoplasm. Cytoplasmic filaments. - Information by UniProt
-
Database links
- Entrez Gene: 58484 Human
- Entrez Gene: 268973 Mouse
- Entrez Gene: 298784 Rat
- Omim: 606831 Human
- SwissProt: Q9NPP4 Human
- SwissProt: Q3UP24 Mouse
- SwissProt: F1M649 Rat
- Unigene: 574741 Human
-
Alternative names
- and NACHT-containing protein antibody
- CARD 12 antibody
- CARD antibody
see all
Images
-
Western blot - Anti-NLRC4 antibody (ab99860)Anti-NLRC4 antibody (ab99860) at 1/500 dilution + Human fetal brain lysate
Predicted band size: 116 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC4 antibody (ab99860)Immunohistochemical staining of formalin-fixed paraffin-embedded human fetal lung showing cytoplasmic staining with ab99860 at a dilution of 1/100.
References
This product has been referenced in:
- Lin ZH et al. Methylene Blue Mitigates Acute Neuroinflammation after Spinal Cord Injury through Inhibiting NLRP3 Inflammasome Activation in Microglia. Front Cell Neurosci 11:391 (2017). Read more (PubMed: 29311826) »
- Ataide MA et al. Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection. PLoS Pathog 10:e1003885 (2014). Read more (PubMed: 24453977) »
