Recombinant Anti-NLRC4 antibody [EPR19733] (ab201792)
![Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)](https://www.abcam.com/ps/products/201/ab201792/Images/ab201792-296682-anti-card12-antibody-epr19733-western-blot.jpg "Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19733] to NLRC4
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-NLRC4 antibody [EPR19733]
See all NLRC4 primary antibodies -
Description
Rabbit monoclonal [EPR19733] to NLRC4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Mouse NLRC4 aa 1-300. The exact sequence is proprietary.
Database link: Q3UP24 -
Positive control
- WB: 293T whole cell lysate transfected with mouse NLRC4; RAW 264.7 overexpressed with ASC whole cell lysate.
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General notes
This product was previously labelled as CARD12
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19733 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab201792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa). |
Target
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Function
Plays a role in the promotion of apoptosis. -
Tissue specificity
Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain. -
Sequence similarities
Contains 1 CARD domain.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Cytoplasm. Cytoplasmic filaments. - Information by UniProt
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Database links
- Entrez Gene: 58484 Human
- Entrez Gene: 268973 Mouse
- Omim: 606831 Human
- SwissProt: Q9NPP4 Human
- SwissProt: Q3UP24 Mouse
- Unigene: 574741 Human
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Alternative names
- and NACHT-containing protein antibody
- CARD 12 antibody
- CARD antibody
see all
Images
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Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/200 dilution + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)](https://www.abcam.com/ps/products/201/ab201792/Images/ab201792-270562-anti-card12-antibody-epr19733-western-blot.jpg)
Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)All lanes : Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution
Lane 1 : 293T whole cell lysate (Human epithelial cell line from embryonic kidney)
Lane 2 : 293T whole cell lysate transfected with mouse NLRC4
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 0.5 second.
The cell lysates were kindly provided by our collaborator Dr. Jiahuai Han, Xiamen University
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![Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)](https://www.abcam.com/ps/products/201/ab201792/Images/ab201792-296681-anti-card12-antibody-epr19733-western-blot.jpg)
Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution + Mouse spleen tissue lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
-
![Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)](https://www.abcam.com/ps/products/201/ab201792/Images/ab201792-270561-anti-card12-antibody-epr19733-western-blot.jpg)
Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)All lanes : Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution
Lane 1 : RAW 264.7 whole cell lysate (Mouse macrophage cell line transformed with Abelson murine leukemia virus) overexpressed with ASC
Lane 2 : RAW 264.7 whole cell lysate NLRC4 KO overexpressed with ASC
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
NLRC4 regulation involves mechanisms that require ASC (apoptosis-associated speck-like protein containing a CARD), a common adaptor protein for several inflammasomes - PMID: 25775556.
The cell lysates were kindly provided by our Collaborator Dr. Jiahuai Han, Xiamen University -
![Anti-NLRC4 antibody [EPR19733] (ab201792)](https://www.abcam.com/ps/products/201/ab201792/Images/ab201792-6-benefits-of-recombinant-antibodies.png)
Anti-NLRC4 antibody [EPR19733] (ab201792)
Datasheets and documents
Certificate of Compliance
References (1)
ab201792 has been referenced in 1 publication.
- Guo Y et al. Essential role of Salmonella Enteritidis DNA adenine methylase in modulating inflammasome activation. BMC Microbiol 20:226 (2020). PubMed: 32723297
