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  1. Link

    nlrc4-antibody-epr19733-ab201792.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-NLRC4 antibody [EPR19733] (ab201792)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (7)

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Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
  • Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
  • Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
  • Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
  • Anti-NLRC4 antibody [EPR19733] (ab201792)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19733] to NLRC4
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Recombinant Human NLRC4 protein (ab127060)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-NLRC4 antibody [EPR19733]
    See all NLRC4 primary antibodies
  • Description

    Rabbit monoclonal [EPR19733] to NLRC4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T whole cell lysate transfected with mouse NLRC4; RAW 264.7 overexpressed with ASC whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19733
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • CARD Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-NLRC4 antibody [EPR19733] - BSA and Azide free (ab223143)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human NLRC4 protein (ab127060)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab201792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa).
Notes
WB
1/1000. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa).

Target

  • Function

    Plays a role in the promotion of apoptosis.
  • Tissue specificity

    Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.
  • Sequence similarities

    Contains 1 CARD domain.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Cellular localization

    Cytoplasm. Cytoplasmic filaments.
  • Target information above from: UniProt accession Q9NPP4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 58484 Human
    • Entrez Gene: 268973 Mouse
    • Omim: 606831 Human
    • SwissProt: Q9NPP4 Human
    • SwissProt: Q3UP24 Mouse
    • Unigene: 574741 Human
    • Alternative names

      • and NACHT-containing protein antibody
      • CARD 12 antibody
      • CARD antibody
      • CARD LRR and NACHT containing protein 1 antibody
      • CARD LRR and NACHT containing protein antibody
      • CARD12 antibody
      • Caspase recruitment domain containing protein 12 antibody
      • Caspase recruitment domain family member 12 antibody
      • Caspase recruitment domain-containing protein 12 antibody
      • CLAN 1 antibody
      • CLAN A antibody
      • CLAN antibody
      • CLAN B antibody
      • CLAN C antibody
      • CLAN D antibody
      • Clan protein antibody
      • CLAN1 antibody
      • CLANA antibody
      • CLANB antibody
      • CLANC antibody
      • CLAND antibody
      • CLR 2.1 antibody
      • CLR2.1 antibody
      • ICE protease activating factor antibody
      • Ice protease-activating factor antibody
      • Ipaf antibody
      • LRR antibody
      • NLR family CARD domain containing 4 antibody
      • NLR family CARD domain-containing protein 4 antibody
      • NLRC 4 antibody
      • NLRC4 antibody
      • NLRC4_HUMAN antibody
      • NOD like receptor C4 antibody
      • Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 4 antibody
      • UNQ6189/PRO20215 antibody
      see all

    Images

    • Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
      Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
      Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/200 dilution + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 117 kDa
      Observed band size: 117 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer: 5% NFDM/TBST

    • Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
      Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
      All lanes : Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution

      Lane 1 : 293T whole cell lysate (Human epithelial cell line from embryonic kidney)
      Lane 2 : 293T whole cell lysate transfected with mouse NLRC4

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 117 kDa
      Observed band size: 117 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 0.5 second.

      The cell lysates were kindly provided by our collaborator Dr. Jiahuai Han, Xiamen University

    • Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
      Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
      Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution + Mouse spleen tissue lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 117 kDa
      Observed band size: 117 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer: 5% NFDM/TBST

    • Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
      Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
      All lanes : Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution

      Lane 1 : RAW 264.7 whole cell lysate (Mouse macrophage cell line transformed with Abelson murine leukemia virus) overexpressed with ASC
      Lane 2 : RAW 264.7 whole cell lysate NLRC4 KO overexpressed with ASC

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 117 kDa
      Observed band size: 117 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      NLRC4 regulation involves mechanisms that require ASC (apoptosis-associated speck-like protein containing a CARD), a common adaptor protein for several inflammasomes - PMID: 25775556.
      The cell lysates were kindly provided by our Collaborator Dr. Jiahuai Han, Xiamen University

    • Anti-NLRC4 antibody [EPR19733] (ab201792)
      Anti-NLRC4 antibody [EPR19733] (ab201792)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (7)

    Publishing research using ab201792? Please let us know so that we can cite the reference in this datasheet.

    ab201792 has been referenced in 7 publications.

    • Wang SS  et al. NLRC4 gene silencing-dependent blockade of NOD-like receptor pathway inhibits inflammation, reduces proliferation and increases apoptosis of dendritic cells in mice with septic shock. Aging (Albany NY) 13:1440-1457 (2021). PubMed: 33406504
    • Wang W  et al. Galectin-9 Targets NLRP3 for Autophagic Degradation to Limit Inflammation. J Immunol 206:2692-2699 (2021). PubMed: 33963043
    • Zhang L  et al. Dexmedetomidine Mitigated NLRP3-Mediated Neuroinflammation via the Ubiquitin-Autophagy Pathway to Improve Perioperative Neurocognitive Disorder in Mice. Front Pharmacol 12:646265 (2021). PubMed: 34079457
    • Zhou L  et al. Salmonella spvC Gene Inhibits Autophagy of Host Cells and Suppresses NLRP3 as Well as NLRC4. Front Immunol 12:639019 (2021). PubMed: 34335562
    • Guo Y  et al. Essential role of Salmonella Enteritidis DNA adenine methylase in modulating inflammasome activation. BMC Microbiol 20:226 (2020). PubMed: 32723297
    • Johnson DC  et al. DPP8/9 inhibitors activate the CARD8 inflammasome in resting lymphocytes. Cell Death Dis 11:628 (2020). PubMed: 32796818
    • Chen S  et al. Serine Supports IL-1ß Production in Macrophages Through mTOR Signaling. Front Immunol 11:1866 (2020). PubMed: 32973770

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