Recombinant Anti-NLRC4 antibody [EPR19733] (ab201792)
![Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)](https://www.abcam.com/ps/products/201/ab201792/Images/ab201792-296682-anti-card12-antibody-epr19733-western-blot.jpg "Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19733] to NLRC4
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-NLRC4 antibody [EPR19733]
See all NLRC4 primary antibodies -
Description
Rabbit monoclonal [EPR19733] to NLRC4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T whole cell lysate transfected with mouse NLRC4; RAW 264.7 overexpressed with ASC whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19733 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa). |
Target
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Function
Plays a role in the promotion of apoptosis. -
Tissue specificity
Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain. -
Sequence similarities
Contains 1 CARD domain.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Cytoplasm. Cytoplasmic filaments. - Information by UniProt
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Database links
- Entrez Gene: 58484 Human
- Entrez Gene: 268973 Mouse
- Omim: 606831 Human
- SwissProt: Q9NPP4 Human
- SwissProt: Q3UP24 Mouse
- Unigene: 574741 Human
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Alternative names
- and NACHT-containing protein antibody
- CARD 12 antibody
- CARD antibody
see all
Images
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Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/200 dilution + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)](https://www.abcam.com/ps/products/201/ab201792/Images/ab201792-270562-anti-card12-antibody-epr19733-western-blot.jpg)
Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)All lanes : Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution
Lane 1 : 293T whole cell lysate (Human epithelial cell line from embryonic kidney)
Lane 2 : 293T whole cell lysate transfected with mouse NLRC4
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 0.5 second.
The cell lysates were kindly provided by our collaborator Dr. Jiahuai Han, Xiamen University
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![Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)](https://www.abcam.com/ps/products/201/ab201792/Images/ab201792-296681-anti-card12-antibody-epr19733-western-blot.jpg)
Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution + Mouse spleen tissue lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
-
![Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)](https://www.abcam.com/ps/products/201/ab201792/Images/ab201792-270561-anti-card12-antibody-epr19733-western-blot.jpg)
Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)All lanes : Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution
Lane 1 : RAW 264.7 whole cell lysate (Mouse macrophage cell line transformed with Abelson murine leukemia virus) overexpressed with ASC
Lane 2 : RAW 264.7 whole cell lysate NLRC4 KO overexpressed with ASC
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
NLRC4 regulation involves mechanisms that require ASC (apoptosis-associated speck-like protein containing a CARD), a common adaptor protein for several inflammasomes - PMID: 25775556.
The cell lysates were kindly provided by our Collaborator Dr. Jiahuai Han, Xiamen University -
![Anti-NLRC4 antibody [EPR19733] (ab201792)](https://www.abcam.com/ps/products/201/ab201792/Images/ab201792-6-benefits-of-recombinant-antibodies.png)
Anti-NLRC4 antibody [EPR19733] (ab201792)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (7)
ab201792 has been referenced in 7 publications.
- Wang SS et al. NLRC4 gene silencing-dependent blockade of NOD-like receptor pathway inhibits inflammation, reduces proliferation and increases apoptosis of dendritic cells in mice with septic shock. Aging (Albany NY) 13:1440-1457 (2021). PubMed: 33406504
- Wang W et al. Galectin-9 Targets NLRP3 for Autophagic Degradation to Limit Inflammation. J Immunol 206:2692-2699 (2021). PubMed: 33963043
- Zhang L et al. Dexmedetomidine Mitigated NLRP3-Mediated Neuroinflammation via the Ubiquitin-Autophagy Pathway to Improve Perioperative Neurocognitive Disorder in Mice. Front Pharmacol 12:646265 (2021). PubMed: 34079457
- Zhou L et al. Salmonella spvC Gene Inhibits Autophagy of Host Cells and Suppresses NLRP3 as Well as NLRC4. Front Immunol 12:639019 (2021). PubMed: 34335562
- Guo Y et al. Essential role of Salmonella Enteritidis DNA adenine methylase in modulating inflammasome activation. BMC Microbiol 20:226 (2020). PubMed: 32723297
- Johnson DC et al. DPP8/9 inhibitors activate the CARD8 inflammasome in resting lymphocytes. Cell Death Dis 11:628 (2020). PubMed: 32796818
- Chen S et al. Serine Supports IL-1ß Production in Macrophages Through mTOR Signaling. Front Immunol 11:1866 (2020). PubMed: 32973770