Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-NLRC4 antibody [EPR19733] - BSA and Azide free (ab223143)

Overview

  • Product name

    Anti-NLRC4 antibody [EPR19733] - BSA and Azide free
    See all NLRC4 primary antibodies
  • Description

    Rabbit monoclonal [EPR19733] to NLRC4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment aa 1-300. The exact sequence is proprietary.
    Database link: Q3UP24

  • Positive control

    • WB: 293T whole cell lysate transfected with mouse NLRC4; RAW 264.7 overexpressed with ASC whole cell lysate.
  • General notes

    ab223143 is the carrier-free version of ab201792 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab223143 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as CARD12

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab223143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa).

Target

  • Function

    Plays a role in the promotion of apoptosis.
  • Tissue specificity

    Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.
  • Sequence similarities

    Contains 1 CARD domain.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Cellular localization

    Cytoplasm. Cytoplasmic filaments.
  • Information by UniProt
  • Database links

  • Alternative names

    • and NACHT-containing protein antibody
    • CARD 12 antibody
    • CARD antibody
    • CARD LRR and NACHT containing protein 1 antibody
    • CARD LRR and NACHT containing protein antibody
    • CARD12 antibody
    • Caspase recruitment domain containing protein 12 antibody
    • Caspase recruitment domain family member 12 antibody
    • Caspase recruitment domain-containing protein 12 antibody
    • CLAN 1 antibody
    • CLAN A antibody
    • CLAN antibody
    • CLAN B antibody
    • CLAN C antibody
    • CLAN D antibody
    • Clan protein antibody
    • CLAN1 antibody
    • CLANA antibody
    • CLANB antibody
    • CLANC antibody
    • CLAND antibody
    • CLR 2.1 antibody
    • CLR2.1 antibody
    • ICE protease activating factor antibody
    • Ice protease-activating factor antibody
    • Ipaf antibody
    • LRR antibody
    • NLR family CARD domain containing 4 antibody
    • NLR family CARD domain-containing protein 4 antibody
    • NLRC 4 antibody
    • NLRC4 antibody
    • NLRC4_HUMAN antibody
    • NOD like receptor C4 antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 4 antibody
    • UNQ6189/PRO20215 antibody
    see all

Images

  • All lanes : Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution

    Lane 1 : RAW 264.7 whole cell lysate (Mouse macrophage cell line transformed with Abelson murine leukemia virus) overexpressed with ASC
    Lane 2 : RAW 264.7 whole cell lysate NLRC4 KO overexpressed with ASC

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 117 kDa
    Observed band size: 117 kDa


    Exposure time: 3 minutes


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201792).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    NLRC4 regulation involves mechanisms that require ASC (apoptosis-associated speck-like protein containing a CARD), a common adaptor protein for several inflammasomes - PMID: 25775556.
    The cell lysates were kindly provided by our Collaborator Dr. Jiahuai Han, Xiamen University

References

ab223143 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223143.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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