Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19733] to NLRC4 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Product nameAnti-NLRC4 antibody [EPR19733] - BSA and Azide free
See all NLRC4 primary antibodies
DescriptionRabbit monoclonal [EPR19733] to NLRC4 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Recombinant fragment aa 1-300. The exact sequence is proprietary.
Database link: Q3UP24
- WB: 293T whole cell lysate transfected with mouse NLRC4; RAW 264.7 overexpressed with ASC whole cell lysate.
ab223143 is the carrier-free version of ab201792 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab223143 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as CARD12
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab223143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa).|
FunctionPlays a role in the promotion of apoptosis.
Tissue specificityIsoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.
Sequence similaritiesContains 1 CARD domain.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.
Cellular localizationCytoplasm. Cytoplasmic filaments.
- Information by UniProt
- and NACHT-containing protein antibody
- CARD 12 antibody
- CARD antibody
All lanes : Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution
Lane 1 : RAW 264.7 whole cell lysate (Mouse macrophage cell line transformed with Abelson murine leukemia virus) overexpressed with ASC
Lane 2 : RAW 264.7 whole cell lysate NLRC4 KO overexpressed with ASC
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 3 minutes
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201792).
Blocking/Dilution buffer: 5% NFDM/TBST.
NLRC4 regulation involves mechanisms that require ASC (apoptosis-associated speck-like protein containing a CARD), a common adaptor protein for several inflammasomes - PMID: 25775556.
The cell lysates were kindly provided by our Collaborator Dr. Jiahuai Han, Xiamen University
ab223143 has not yet been referenced specifically in any publications.