Anti-NLRP3 antibody (ab214185)
Key features and details
- Rabbit polyclonal to NLRP3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NLRP3 antibody
See all NLRP3 primary antibodies -
Description
Rabbit polyclonal to NLRP3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human NLRP3 aa 1-50 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:MKMASTRCKL ARYLEDLEDV DLKKFKMHLE DYPPQKGCIP LPRGQTEKAD
Database link: Q96P20 -
Positive control
- IHC-P: Human lung carcinoma tissue. Mouse intestine and embryo tissue. Rat spinal cord and kidney tissue. WB: Raji, SiHa and MDA-MB-231 cell lysate, mouse spleen and thymus tissue lysate.
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General notes
The level of NLRP3 expression can be elevated when cells are treated with LPS, as described in the literature (PMID: 22569257).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab214185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
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| WB | 1/100 - 1/1000. The level of NLRP3 expression can be elevated when cells are treated with LPS. There may be multiple bands of NLRP3 in WB assay due to different isoforms and PTMs. Hu Isoform I: 84kD (predicted) Isoform 2-6: 106-120kD (predicted) Ms Isoform I: 96kD (predicted) Isoform 2-4: 110-120kD (predicted) Positive Control: Human THP1 WT cells treated with 200 ng/ml LPS for 3h, RAW 264.7 (+/- treatment with LPS) ab7187, Rat spleen lysate. Negative Control: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate. |
Target
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Function
May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. -
Tissue specificity
Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. -
Involvement in disease
Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. -
Sequence similarities
Belongs to the NLRP family.
Contains 1 DAPIN domain.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 114548 Human
- Entrez Gene: 216799 Mouse
- Entrez Gene: 287362 Rat
- Omim: 606416 Human
- SwissProt: Q96P20 Human
- SwissProt: Q8R4B8 Mouse
- Unigene: 159483 Human
- Unigene: 54174 Mouse
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Alternative names
- AGTAVPRL antibody
- AII/AVP antibody
- Angiotensin/vasopressin receptor AII/AVP like antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse intestine tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.
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Western blot - Anti-NLRP3 antibody (ab214185)All lanes : Anti-NLRP3 antibody (ab214185) at 1/300 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line/0 cell lysate
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG at 1/5000 dilution -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse embryo tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.
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Western blot - Anti-NLRP3 antibody (ab214185)All lanes : Anti-NLRP3 antibody (ab214185) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse thymus tissue lysate
Secondary
All lanes : Conjugated secondary antibody at 1/20000 dilution -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human lung carcinoma tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse intestine tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat kidney tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.
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Western blot - Anti-NLRP3 antibody (ab214185)Anti-NLRP3 antibody (ab214185) at 1/300 dilution + SiHa (Human cervical carcinoma cell line) cell lysate
Secondary
HRP-conjugated goat anti-rabbit IgG
Protocols
References (109)
ab214185 has been referenced in 109 publications.
- Qi S et al. P38 MAPK signaling pathway mediates COM crystal-induced crystal adhesion change in rat renal tubular epithelial cells. Urolithiasis 48:9-18 (2020). PubMed: 31183507
- Iida T et al. Down-regulation of RalGTPase-Activating Protein Promotes Colitis-Associated Cancer via NLRP3 Inflammasome Activation. Cell Mol Gastroenterol Hepatol 9:277-293 (2020). PubMed: 31622786
- Dong F et al. miR-20b inhibits the senescence of human umbilical vein endothelial cells through regulating the Wnt/ß-catenin pathway via the TXNIP/NLRP3 axis. Int J Mol Med 45:847-857 (2020). PubMed: 31922218
- Yu J et al. Ezetimibe Attenuates Oxidative Stress and Neuroinflammation via the AMPK/Nrf2/TXNIP Pathway after MCAO in Rats. Oxid Med Cell Longev 2020:4717258 (2020). PubMed: 31998437
- Fu L et al. Exogenous carbon monoxide protects against mitochondrial DNA-induced hippocampal pyroptosis in a model of hemorrhagic shock and resuscitation. Int J Mol Med 45:1176-1186 (2020). PubMed: 32124959
