For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    nlrp3-antibody-ab214185.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Macrophage / Inflamm.
Share by email

Anti-NLRP3 antibody (ab214185)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (109)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
  • Western blot - Anti-NLRP3 antibody (ab214185)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
  • Western blot - Anti-NLRP3 antibody (ab214185)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
  • Western blot - Anti-NLRP3 antibody (ab214185)

Key features and details

  • Rabbit polyclonal to NLRP3
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human NLRP3 protein (ab165022)
Primary
Product image
Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-NLRP3 antibody
    See all NLRP3 primary antibodies
  • Description

    Rabbit polyclonal to NLRP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NLRP3 aa 1-50 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    MKMASTRCKL ARYLEDLEDV DLKKFKMHLE DYPPQKGCIP LPRGQTEKAD


    Database link: Q96P20
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human lung carcinoma tissue. Mouse intestine and embryo tissue. Rat spinal cord and kidney tissue. WB: Raji, SiHa and MDA-MB-231 cell lysate, mouse spleen and thymus tissue lysate.
  • General notes

    The level of NLRP3 expression can be elevated when cells are treated with LPS, as described in the literature (PMID: 22569257). 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cancer
    • Growth factors
    • TNF
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
  • Recombinant Protein

    • Recombinant Human NLRP3 protein (ab165022)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Recombinant Human NLRP3 protein (ab165022)

Applications

Our Abpromise guarantee covers the use of ab214185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

WB 1/100 - 1/1000.

The level of NLRP3 expression can be elevated when cells are treated with LPS. There may be multiple bands of NLRP3 in WB assay due to different isoforms and PTMs.

Hu Isoform I: 84kD (predicted) Isoform 2-6: 106-120kD (predicted)

Ms Isoform I: 96kD (predicted) Isoform 2-4: 110-120kD (predicted)

Positive Control: Human THP1 WT cells treated with 200 ng/ml LPS for 3h, RAW 264.7 (+/- treatment with LPS) ab7187, Rat spleen lysate.

Negative Control: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate.

Target

  • Function

    May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
  • Tissue specificity

    Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
  • Involvement in disease

    Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
    Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
    Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.
  • Sequence similarities

    Belongs to the NLRP family.
    Contains 1 DAPIN domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q96P20 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 114548 Human
    • Entrez Gene: 216799 Mouse
    • Entrez Gene: 287362 Rat
    • Omim: 606416 Human
    • SwissProt: Q96P20 Human
    • SwissProt: Q8R4B8 Mouse
    • Unigene: 159483 Human
    • Unigene: 54174 Mouse
    • Alternative names

      • AGTAVPRL antibody
      • AII/AVP antibody
      • Angiotensin/vasopressin receptor AII/AVP like antibody
      • Angiotensin/vasopressin receptor AII/AVP-like antibody
      • C1orf7 antibody
      • Caterpiller protein 1.1 antibody
      • CIAS 1 antibody
      • CIAS1 antibody
      • CLR1.1 antibody
      • Cold autoinflammatory syndrome 1 antibody
      • Cold autoinflammatory syndrome 1 protein antibody
      • Cryopyrin antibody
      • Familial cold autoinflammatory syndrome antibody
      • FCAS antibody
      • FCU antibody
      • LRR and PYD domains-containing protein 3 antibody
      • Muckle-Wells syndrome antibody
      • MWS antibody
      • NACHT antibody
      • NACHT LRR and PYD containing protein 3 antibody
      • NALP 3 antibody
      • NALP3 antibody
      • NALP3_HUMAN antibody
      • NLR family pyrin domain containing 3 antibody
      • NLRP3 antibody
      • PYPAF 1 antibody
      • PYPAF1 antibody
      • PYRIN containing APAF1 like protein 1 antibody
      • PYRIN-containing APAF1-like protein 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)

      Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse intestine tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.

    • Western blot - Anti-NLRP3 antibody (ab214185)
      Western blot - Anti-NLRP3 antibody (ab214185)
      All lanes : Anti-NLRP3 antibody (ab214185) at 1/300 dilution

      Lane 1 : Raji (human Burkitt's lymphoma cell line) cell lysate
      Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line/0 cell lysate

      Secondary
      All lanes : HRP conjugated goat anti-rabbit IgG at 1/5000 dilution
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)

      Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse embryo tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.

    • Western blot - Anti-NLRP3 antibody (ab214185)
      Western blot - Anti-NLRP3 antibody (ab214185)
      All lanes : Anti-NLRP3 antibody (ab214185) at 1/1000 dilution

      Lane 1 : Mouse spleen tissue lysate
      Lane 2 : Mouse thymus tissue lysate

      Secondary
      All lanes : Conjugated secondary antibody at 1/20000 dilution
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)

      Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human lung carcinoma tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)

      Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse intestine tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRP3 antibody (ab214185)

      Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat kidney tissue sections labeling NLRP3 using ab214185 at a 1/200 dilution. DAB staining.

    • Western blot - Anti-NLRP3 antibody (ab214185)
      Western blot - Anti-NLRP3 antibody (ab214185)
      Anti-NLRP3 antibody (ab214185) at 1/300 dilution + SiHa (Human cervical carcinoma cell line) cell lysate

      Secondary
      HRP-conjugated goat anti-rabbit IgG

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (109)

    Publishing research using ab214185? Please let us know so that we can cite the reference in this datasheet.

    ab214185 has been referenced in 109 publications.

    • Qi S  et al. P38 MAPK signaling pathway mediates COM crystal-induced crystal adhesion change in rat renal tubular epithelial cells. Urolithiasis 48:9-18 (2020). PubMed: 31183507
    • Iida T  et al. Down-regulation of RalGTPase-Activating Protein Promotes Colitis-Associated Cancer via NLRP3 Inflammasome Activation. Cell Mol Gastroenterol Hepatol 9:277-293 (2020). PubMed: 31622786
    • Dong F  et al. miR-20b inhibits the senescence of human umbilical vein endothelial cells through regulating the Wnt/ß-catenin pathway via the TXNIP/NLRP3 axis. Int J Mol Med 45:847-857 (2020). PubMed: 31922218
    • Yu J  et al. Ezetimibe Attenuates Oxidative Stress and Neuroinflammation via the AMPK/Nrf2/TXNIP Pathway after MCAO in Rats. Oxid Med Cell Longev 2020:4717258 (2020). PubMed: 31998437
    • Fu L  et al. Exogenous carbon monoxide protects against mitochondrial DNA-induced hippocampal pyroptosis in a model of hemorrhagic shock and resuscitation. Int J Mol Med 45:1176-1186 (2020). PubMed: 32124959
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-NLRP3 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Lysates of BMDCs)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    25000 cells
    Treatment
    LPS (3h, 50 ng/ml)
    Specification
    Lysates of BMDCs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Mr. Benedikt Saller

    Verified customer

    Submitted Aug 30 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.