• Product name

  • Description

    Goat polyclonal to NLRP3
  • Host species

  • Tested applications

    Suitable for: Flow Cyt, IHC-FoFr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human NLRP3 aa 1024-1036 (C terminal).


  • General notes

    No signal has been obtained in Western blot but low background has observed in Daudi, A431, Jurkat, U937 and MOLT-4 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field.



Our Abpromise guarantee covers the use of ab4207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-FoFr Use at an assay dependent concentration. PubMed: 28726778
ICC/IF Use at an assay dependent concentration.


  • Function

    May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
  • Tissue specificity

    Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
  • Involvement in disease

    Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
    Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
    Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.
  • Sequence similarities

    Belongs to the NLRP family.
    Contains 1 DAPIN domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • AGTAVPRL antibody
    • AII/AVP antibody
    • Angiotensin/vasopressin receptor AII/AVP like antibody
    • Angiotensin/vasopressin receptor AII/AVP-like antibody
    • C1orf7 antibody
    • Caterpiller protein 1.1 antibody
    • CIAS 1 antibody
    • CIAS1 antibody
    • CLR1.1 antibody
    • Cold autoinflammatory syndrome 1 antibody
    • Cold autoinflammatory syndrome 1 protein antibody
    • Cryopyrin antibody
    • Familial cold autoinflammatory syndrome antibody
    • FCAS antibody
    • FCU antibody
    • LRR and PYD domains-containing protein 3 antibody
    • Muckle-Wells syndrome antibody
    • MWS antibody
    • NACHT antibody
    • NACHT LRR and PYD containing protein 3 antibody
    • NALP 3 antibody
    • NALP3 antibody
    • NALP3_HUMAN antibody
    • NLR family pyrin domain containing 3 antibody
    • NLRP3 antibody
    • PYPAF 1 antibody
    • PYPAF1 antibody
    • PYRIN containing APAF1 like protein 1 antibody
    • PYRIN-containing APAF1-like protein 1 antibody
    see all


  • Effects of ATP, cholesterol crystals and 7-Ketocholesterol on Nlrp3 inflammasome formation in Asc+/+and Asc−/− BMMs

    Representative confocal fluorescence images depicting the effects of cholesterol crystals (CHC, 0.5 mg/ml, 16 h), 7-ketocholesterol (7-Ket, 10 µg/ml, 16 h), or ATP (2.5 mM, 16 h) on the colocalization between Nlrp3 and Asc from Asc+/+ and Asc−/− BMMs.

    4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse bone marrow macrophages were stained for NLRP3 using ab4207 at 1/200 dilution in ICC/IF.

    (From Figure 2A of Li et al)

  • Paraformaldehyde-fixed, 0.15% Triton X-100 permeabilized U937 (human histiocytic lymphoma cell line) cells stained for NLRP3 (green) using ab4207 at 10 μg/ml in ICC/IF. (green). The nuclear counterstain is DAPI (blue). The negative control is unimmunized goaet IgG at 10 μug/ml, followed by the secndary antibody.

  • Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized human PBMCs labeling NLRP3 with ab4207 at 10 μg/ml (blue) compared to an unimmunized control IgG (black). Gated on granulocytes.

  • ab4207 staining NLRP3 in the Human White Blood Cells (Mixed Population) by Flow Cytometry. WBC were isolated spinning Blood on Ficoll Gradient after removal of RBC's and permeabilized with 0.1% Triton-X100 in 2% BSA for 15 minutes. The sample was incubated with the primary antibody (1/100 in PBS + 2% BSA in PBS) for 16 hours at 4°C. An Alexa Flour488 Donkey Anti Goat IgG (H+L) (1/250) was used as the secondary antibody.
    Gating Strategy: Monocytes

  • ab4207 staining CIAS1 / NALP3 in murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab4207 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor® 488 conjugated donkey anti-goat (H+L) polyclonal used at a 1/500 dilution.WGA: Alexa Fluor® 568 Wheat Germ Agglutinin.

    See Abreview

  • Immunofluorescence analysis of Human MDM cells, staining CIAS1 / NALP3 with ab4207.
    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% goat serum for 1 hour at room temp. Samples were incubated with primary antibody (1/200 in 1% BSA in PBS) for 12 hours at 4°C. A Cy5® conjugated donkey anti-goat polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview


This product has been referenced in:

  • Villella VR  et al. A pathogenic role for cystic fibrosis transmembrane conductance regulator in celiac disease. EMBO J 38:N/A (2019). Read more (PubMed: 30498130) »
  • Luo Y  et al. MCC950 attenuated early brain injury by suppressing NLRP3 inflammasome after experimental SAH in rats. Brain Res Bull 146:320-326 (2019). Read more (PubMed: 30716395) »
See all 44 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Immunohistochemistry (Frozen sections)
Rat Tissue sections (Brain)
Yes - 5% Triton-X in 0.05M Phosphate Buffered Saline
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Feb 28 2018

Western blot
Human Cell lysate - whole cell (macrophage)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
0.1 ug/ml LPS 15 min
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 12 2014

Immunocytochemistry/ Immunofluorescence
Human Cell (human MDM)
human MDM
Yes - TRITON-X (0.1 %)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 06 2013


DISCOUNT CODE: *** Expiration date: February 29th, 2013 Value: *** I am very pleased to hear you would like to accept our offer and test ab4207 in canine. This code will give you *** off your next order before the expiration date. To redeem this offer, please submit an Abreview for canine and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Read More
Immunocytochemistry/ Immunofluorescence
Mouse Cell (RAW 264.7)
RAW 264.7
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted May 24 2012

Western blot
Rat Tissue lysate - whole (Corpus Cavernosum)
Loading amount
20 µg
Corpus Cavernosum
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Jason Davis

Verified customer

Submitted Jan 13 2012

Flow Cytometry
Human Cell (White Blood Cells (Mixed Population))
White Blood Cells (Mixed Population)
Cell harvesting/tissue preparation method: WBC were isolated spinning Blood on Ficoll Gradient after removal of RBC's
Sample buffer: PBS
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy

Dr. Mahesh Shivananjappa

Verified customer

Submitted Feb 15 2011

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