Key features and details
- Goat polyclonal to NLRP3
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NLRP3 antibody
See all NLRP3 primary antibodies
DescriptionGoat polyclonal to NLRP3
Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Cow
- ICC/IF: U937 cells; Human MDM cells. Flow Cyt: Human peripheral blood monocytes.
No signal has been obtained in Western blot but low background has observed in Daudi, A431, Jurkat, U937 and MOLT-4 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab4207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 10 µg/ml.
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionMay function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
Tissue specificityExpressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
Involvement in diseaseDefects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.
Sequence similaritiesBelongs to the NLRP family.
Contains 1 DAPIN domain.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.
- Information by UniProt
- AGTAVPRL antibody
- AII/AVP antibody
- Angiotensin/vasopressin receptor AII/AVP like antibody
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Incubated with the primary antibody (ab4207) for 1hr at 10µg/ml, followed by Alexa Fluor® 488 secondary antibody at 2µg/ml. Shows cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor® 488 secondary antibody (2µg/ml).
ab4207 staining NLRP3 in the Human White Blood Cells (Mixed Population) by Flow Cytometry. WBC were isolated spinning Blood on Ficoll Gradient after removal of RBC's and permeabilized with 0.1% Triton-X100 in 2% BSA for 15 minutes. The sample was incubated with the primary antibody (1/100 in PBS + 2% BSA in PBS) for 16 hours at 4°C. An Alexa Flour® 488 Donkey Anti Goat IgG (H+L) (1/250) was used as the secondary antibody.
Gating Strategy: Monocytes
Immunofluorescence analysis of Human MDM cells, staining CIAS1 / NALP3 with ab4207.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% goat serum for 1 hour at room temp. Samples were incubated with primary antibody (1/200 in 1% BSA in PBS) for 12 hours at 4°C. A Cy5® conjugated donkey anti-goat polyclonal IgG (1/400) was used as the secondary antibody.
ab4207 has been referenced in 56 publications.
- Garré JM et al. P2X7 receptor inhibition ameliorates dendritic spine pathology and social behavioral deficits in Rett syndrome mice. Nat Commun 11:1784 (2020). PubMed: 32286307
- Yan S et al. Pharmacological Inhibition of HDAC6 Attenuates NLRP3 Inflammatory Response and Protects Dopaminergic Neurons in Experimental Models of Parkinson's Disease. Front Aging Neurosci 12:78 (2020). PubMed: 32296327
- Wang Y et al. Mitochondrial ROS promote macrophage pyroptosis by inducing GSDMD oxidation. J Mol Cell Biol 11:1069-1082 (2019). PubMed: 30860577
- Javanmard Khameneh H et al. The Inflammasome Adaptor ASC Intrinsically Limits CD4+ T-Cell Proliferation to Help Maintain Intestinal Homeostasis. Front Immunol 10:1566 (2019). PubMed: 31379813
- Dumont A et al. Docosahexaenoic acid inhibits both NLRP3 inflammasome assembly and JNK-mediated mature IL-1ß secretion in 5-fluorouracil-treated MDSC: implication in cancer treatment. Cell Death Dis 10:485 (2019). PubMed: 31217433