Anti-NLRP3 antibody (ab4207)
Key features and details
- Goat polyclonal to NLRP3
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-NLRP3 antibody
See all NLRP3 primary antibodies -
Description
Goat polyclonal to NLRP3 -
Host species
Goat -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow -
Immunogen
Synthetic peptide corresponding to Human NLRP3 aa 1024-1036 (C terminal).
Sequence:C-EKPELTVVFEPSW
Database link: Q96P20-1 -
Positive control
- Flow Cyt (intra): U937 cells. WB: THP-1 cells ICC/IF: U937cells
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General notes
No signal has been obtained in Western blot but low background has observed in Daudi, A431, Jurkat, U937 and MOLT-4 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (3) |
Use a concentration of 10 µg/ml.
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WB | (2) |
1/1000. Predicted molecular weight: 118 kDa.
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Notes |
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Flow Cyt (Intra)
Use a concentration of 10 µg/ml. ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 10 µg/ml. |
WB
1/1000. Predicted molecular weight: 118 kDa. |
Target
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Function
May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. -
Tissue specificity
Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. -
Involvement in disease
Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. -
Sequence similarities
Belongs to the NLRP family.
Contains 1 DAPIN domain.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 114548 Human
- Entrez Gene: 287362 Rat
- Omim: 606416 Human
- SwissProt: Q96P20 Human
- Unigene: 159483 Human
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Alternative names
- AGTAVPRL antibody
- AII/AVP antibody
- Angiotensin/vasopressin receptor AII/AVP like antibody
see all
Images
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All lanes : Anti-NLRP3 antibody (ab4207) at 1/1000 dilution
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : NLRP3 knockout THP-1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDaFalse colour image of Western blot: Anti-NLRP3 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab4207 was shown to bind specifically to NLRP3. A band was observed at 118 kDa in wild-type THP-1 cell lysates with no signal observed at this size in NLRP3 knockout cell line ab280063 (knockout cell lysate ab280122). To generate this image, wild-type and NLRP3 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216778) at 1/20000 dilution.
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Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
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Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab4207 was 1hr (10ug/ml) followed by Alexa Fluor® 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
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ab4207 staining NLRP3 in the Human White Blood Cells (Mixed Population) by Flow Cytometry. WBC were isolated spinning Blood on Ficoll Gradient after removal of RBC's and permeabilized with 0.1% Triton-X100 in 2% BSA for 15 minutes. The sample was incubated with the primary antibody (1/100 in PBS + 2% BSA in PBS) for 16 hours at 4°C. An Alexa Flour® 488 Donkey Anti Goat IgG (H+L) (1/250) was used as the secondary antibody.
Gating Strategy: Monocytes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (110)
ab4207 has been referenced in 110 publications.
- Shen J et al. TLR9 regulates NLRP3 inflammasome activation via the NF-kB signaling pathway in diabetic nephropathy. Diabetol Metab Syndr 14:26 (2022). PubMed: 35120573
- Lee HJ et al. Idebenone Regulates Aβ and LPS-Induced Neurogliosis and Cognitive Function Through Inhibition of NLRP3 Inflammasome/IL-1β Axis Activation. Front Immunol 13:749336 (2022). PubMed: 35222363
- Lian D et al. Kakonein restores hyperglycemia-induced macrophage digestion dysfunction through regulation of cathepsin B-dependent NLRP3 inflammasome activation. J Leukoc Biol 112:143-155 (2022). PubMed: 35224772
- Xu Q et al. NLRP3 Knockout Protects against Lung Injury Induced by Cerebral Ischemia-Reperfusion. Oxid Med Cell Longev 2022:6260102 (2022). PubMed: 35432726
- Guo P et al. Eriodictyol and Homoeriodictyol Improve Memory Impairment in Aβ25-35-Induced Mice by Inhibiting the NLRP3 Inflammasome. Molecules 27:N/A (2022). PubMed: 35458684