Overview

  • Product name

  • Description

    Rabbit polyclonal to NLRP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human NLRP3 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control

    • MDA-MB231 cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab91413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: Isoform 2, 118 kDa; Isoform 1, 106 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
    • Tissue specificity

      Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
    • Involvement in disease

      Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
      Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
      Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.
    • Sequence similarities

      Belongs to the NLRP family.
      Contains 1 DAPIN domain.
      Contains 9 LRR (leucine-rich) repeats.
      Contains 1 NACHT domain.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • AGTAVPRL antibody
      • AII/AVP antibody
      • Angiotensin/vasopressin receptor AII/AVP like antibody
      • Angiotensin/vasopressin receptor AII/AVP-like antibody
      • C1orf7 antibody
      • Caterpiller protein 1.1 antibody
      • CIAS 1 antibody
      • CIAS1 antibody
      • CLR1.1 antibody
      • Cold autoinflammatory syndrome 1 antibody
      • Cold autoinflammatory syndrome 1 protein antibody
      • Cryopyrin antibody
      • Familial cold autoinflammatory syndrome antibody
      • FCAS antibody
      • FCU antibody
      • LRR and PYD domains-containing protein 3 antibody
      • Muckle-Wells syndrome antibody
      • MWS antibody
      • NACHT antibody
      • NACHT LRR and PYD containing protein 3 antibody
      • NALP 3 antibody
      • NALP3 antibody
      • NALP3_HUMAN antibody
      • NLR family pyrin domain containing 3 antibody
      • NLRP3 antibody
      • PYPAF 1 antibody
      • PYPAF1 antibody
      • PYRIN containing APAF1 like protein 1 antibody
      • PYRIN-containing APAF1-like protein 1 antibody
      see all

    Images

    • Anti-NLRP3 antibody (ab91413) at 1/100 dilution + MDA-MB231 cell line lysate at 35 µg

      Predicted band size: Isoform 2, 118 ; Isoform 1, 106 kDa

    References

    This product has been referenced in:

    • Feng J  et al. Unconjugated bilirubin induces pyroptosis in cultured rat cortical astrocytes. J Neuroinflammation 15:23 (2018). Read more (PubMed: 29357878) »
    • Yang X  et al. CD36 Promotes Podocyte Apoptosis by Activating the Pyrin Domain-Containing-3 (NLRP3) Inflammasome in Primary Nephrotic Syndrome. Med Sci Monit 24:6832-6839 (2018). Read more (PubMed: 30258045) »
    See all 4 Publications for this product

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