Overview

  • Product name

    Anti-NLRP3 antibody [EPR20425]
    See all NLRP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR20425] to NLRP3
  • Host species

    Rabbit
  • Specificity

    The level of NLRP3 expression can be elevated when cells are treated with LPS, as described in the literature (PMID: 22569257).

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NLRP3 aa 1-250. The exact sequence is proprietary.
    Database link: Q96P20

  • Positive control

    • WB: Untreated RAW 264.7 whole cell lysate; RAW 264.7 treated with 100 ng/ml lipopolysaccharides (LPS) for 6 hours, whole cell lysate; THP-1 and U937 whole cell lysates; Human thymus lysate; Mouse lung, spleen and thymus lysates; Rat thymus lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab210491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).

Blocking/Dilution buffer: 5% Non-Fat Dry Milk/TBST.

Target

  • Function

    May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
  • Tissue specificity

    Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
  • Involvement in disease

    Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
    Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
    Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.
  • Sequence similarities

    Belongs to the NLRP family.
    Contains 1 DAPIN domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGTAVPRL antibody
    • AII/AVP antibody
    • Angiotensin/vasopressin receptor AII/AVP like antibody
    • Angiotensin/vasopressin receptor AII/AVP-like antibody
    • C1orf7 antibody
    • Caterpiller protein 1.1 antibody
    • CIAS 1 antibody
    • CIAS1 antibody
    • CLR1.1 antibody
    • Cold autoinflammatory syndrome 1 antibody
    • Cold autoinflammatory syndrome 1 protein antibody
    • Cryopyrin antibody
    • Familial cold autoinflammatory syndrome antibody
    • FCAS antibody
    • FCU antibody
    • LRR and PYD domains-containing protein 3 antibody
    • Muckle-Wells syndrome antibody
    • MWS antibody
    • NACHT antibody
    • NACHT LRR and PYD containing protein 3 antibody
    • NALP 3 antibody
    • NALP3 antibody
    • NALP3_HUMAN antibody
    • NLR family pyrin domain containing 3 antibody
    • NLRP3 antibody
    • PYPAF 1 antibody
    • PYPAF1 antibody
    • PYRIN containing APAF1 like protein 1 antibody
    • PYRIN-containing APAF1-like protein 1 antibody
    see all

Images

  • All lanes : Anti-NLRP3 antibody [EPR20425] (ab210491) at 1/5000 dilution

    Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : RAW 264.7 treated with 100 ng/ml lipopolysaccharides (LPS) for 6 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 118 kDa
    Observed band size: 118 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    The level of NLRP3 expression can be elevated when cells are treated with LPS, as described in the literature (PMID: 22569257).

  • All lanes : Anti-NLRP3 antibody [EPR20425] (ab210491) at 1/1000 dilution

    Lane 1 : Mouse Wt BMDCs treated with 50 ng/ml LPS, 3h.
    Lane 2 : Mouse NLRP3-/- BMDCs treated with 50 ng/ml LPS, 3h.
    Lane 3 : Human THP1 Wt cells treated with 200 ng/ml LPS, 3h.

    Lysates/proteins at 25000 cells per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 118 kDa
    Observed band size: 118 kDa


    Exposure time: 1 minute


    Blocking step: 2% SMP in PBS/T for 60 minutes at RT.

  • All lanes : Anti-NLRP3 antibody [EPR20425] (ab210491) at 1/1000 dilution

    Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
    Lane 2 : THP-1 (Human monocytic leukemia monocyte) treated with 100 ng/ml lipopolysaccharides for 3 hours whole cell lysates
    Lane 3 : Mouse lung lysates
    Lane 4 : Mouse thymus lysates
    Lane 5 : Mouse spleen lysates
    Lane 6 : Mouse kidney lysates
    Lane 7 : Rat thymus lysates
    Lane 8 : Rat lung lysates
    Lane 9 : Rat spleen lysates
    Lane 10 : Mouse hippocampus lysates
    Lane 11 : Rat heart lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 118 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST

    Exposure time:

    Lane 1 and 2: 10 seconds

    Lane 3 to 11: 37 seconds

References

This product has been referenced in:

  • Cao W  et al. Homeobox a5 Promotes White Adipose Tissue Browning Through Inhibition of the Tenascin C/Toll-Like Receptor 4/Nuclear Factor Kappa B Inflammatory Signaling in Mice. Front Immunol 9:647 (2018). Read more (PubMed: 29651293) »
  • Yin D  et al. Dexmedetomidine attenuated early brain injury in rats with subarachnoid haemorrhage by suppressing the inflammatory response: The TLR4/NF-?B pathway and the NLRP3 inflammasome may be involved in the mechanism. Brain Res N/A:N/A (2018). WB ; Rat . Read more (PubMed: 29842860) »
See all 8 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.8
Permeabilization
No
Specification
liver
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Formaldehyde

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Verified customer

Submitted Jul 18 2018

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