Overview

Description

  • Nature

    Synthetic

Specifications

Our Abpromise guarantee covers the use of ab139102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • Form

    Liquid

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituent: PBS

General Info

  • Alternative names

    • AGTAVPRL
    • AII/AVP
    • Angiotensin/vasopressin receptor AII/AVP like
    • Angiotensin/vasopressin receptor AII/AVP-like
    • C1orf7
    • Caterpiller protein 1.1
    • CIAS 1
    • CIAS1
    • CLR1.1
    • Cold autoinflammatory syndrome 1
    • Cold autoinflammatory syndrome 1 protein
    • Cryopyrin
    • Familial cold autoinflammatory syndrome
    • FCAS
    • FCU
    • LRR and PYD domains-containing protein 3
    • Muckle-Wells syndrome
    • MWS
    • NACHT
    • NACHT LRR and PYD containing protein 3
    • NALP 3
    • NALP3
    • NALP3_HUMAN
    • NLR family pyrin domain containing 3
    • NLRP3
    • PYPAF 1
    • PYPAF1
    • PYRIN containing APAF1 like protein 1
    • PYRIN-containing APAF1-like protein 1
    see all
  • Function

    May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
  • Tissue specificity

    Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
  • Involvement in disease

    Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
    Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
    Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.
  • Sequence similarities

    Belongs to the NLRP family.
    Contains 1 DAPIN domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt

References

This product has been referenced in:

  • Alfonso-Loeches S  et al. Role of mitochondria ROS generation in ethanol-induced NLRP3 inflammasome activation and cell death in astroglial cells. Front Cell Neurosci 8:216 (2014). Blocking . Read more (PubMed: 25136295) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us with this follow up information.

I am very glad that the peptide helped to determine which bands were correct.


Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for confirming these details and for your cooperation. I have created the order for the CIAS1/NALP3 peptide , ab139102 to be sent to free of charge. Please see below for details.

As requested, I have issued a free of charge replacement with the order number 1200992.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Answer

Thank you for your reply.

I may have found these orders but I did not see you listed on them. To insure that I have the correct information, could you please confirm the PO numbers or the name/email of the person who had ordered these for you?

I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you for contacting us.

The blocking peptide ab139102 for use with anti-CIAS1/NALP3 antibody ab91525 is available in a 50ug size for $166. However if your orignal contact with us regarding this antibody was within the six month Abpromise guarantee period, I would be happy to provide this peptide for you free of charge as goodwill. If you could please provide the Abcam confirmation number for purchase of ab91525 or the PO and date of purchase I can process this peptide order for you.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

I suggest increasing the primary antibody concentration based on the images which show poor bands for the expected size (˜95kDa)as compared to the 70kDa non specific band. I also suggest using BSA as a blocking buffer to attempt to change the conditions for antibody binding and hopefully reduce the non specific band at 70kDa.

While neither of the bands are at the expected size of 118kDa, this expected size has been based off sequence information and may be different in experiment. I note that there are isoforms of this protein with isoform 4 having a predicted 95kDa size,http://www.uniprot.org/uniprot/Q8R4B8

As the 95kDa size is likely CIAS1 / NALP3, it seems that the primary goal should be to increase the specific signal and decrease non specific binding at the 70kDa size. If you agree, I would like to send you the positive control for this product so that you may have a known sample to compare with. Ab91525 has a recommend positive control of K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate - tumor cell line;https://www.abcam.com/K562-Human-erythromyeloblastoid-leukemia-cell-line-Whole-Cell-Lysate-tumor-cell-line-ab29306.html.

We do have the immunogen peptide available for this product, ab139102. There is currently a 1-2 week lead time for this product. I will attached our immunogen blocking protocol to this email for your review.

I hope this information is helpful to you and look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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