Overview

  • Product name

  • Description

    Rabbit polyclonal to NLRP6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human NLRP6 (C terminal).

  • General notes

     This product was previously labelled as NALP6

     

Properties

Applications

Our Abpromise guarantee covers the use of ab58705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

Target

  • Function

    As the sensor component of the NLRP6 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP6, PYCARD and CASP1 (and possibly CASP4 and CASP5). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. The precise NLRP6 activation stimulus has not been identified yet (By similarity) (PubMed:12387869). Essential for gut mucosal self-renewal and proliferation. Maintains intestinal homeostasis and a healthy intestinal microbiota. This function is, at least partially, mediated by IL18, and not IL1B, produced by nonhematopoietic cells. Influences intestinal barrier function and microbial homeostasis through the regulation of goblet cell mucus secretion. Acts by promoting autophagy in goblet cells, an essential step for mucus granule exocytosis. Its role in goblet cell physiology is inflammasome-dependent, but IL1B- and IL18-independent. During systemic bacterial infections, may negatively regulate inflammatory signaling and inhibit the influx of monocytes and neutrophils to the circulation and to the peritoneum. May promote peripheral nerve recovery following injury via an inflammasome-independent mechanism.
  • Tissue specificity

    Expressed in peripheral blood leukocytes, predominantly in granulocytes and, at lower levels, in CD4(+) and CD8(+) T-cells. Expressed in colonic myofibroblasts (at protein level) (PubMed:21593405).
  • Sequence similarities

    Belongs to the NLRP family.
    Contains 5 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
    Contains 1 pyrin domain.
  • Cellular localization

    Cytoplasm. Inflammasome. Cell membrane. Nucleus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Angiotensin II/vasopressin receptor antibody
    • AVR antibody
    • CLR11.4 antibody
    • NACHT, leucine rich repeat and PYD containing 6 antibody
    • NACHT, LRR and PYD containing protein 6 antibody
    • NACHT, LRR and PYD domains-containing protein 6 antibody
    • NAVR antibody
    • NAVR/AVR antibody
    • NLR family, pyrin domain containing 6 antibody
    • Nlrp6 antibody
    • NLRP6_HUMAN antibody
    • Nucleotide binding oligomerization domain, leucine rich repeat and pyrin domain containing 6 antibody
    • PAN3 antibody
    • PYPAF5 antibody
    • PYRIN containing APAF1 like protein 5 antibody
    • PYRIN-containing APAF1-like protein 5 antibody
    see all

References

This product has been referenced in:

  • Lendermon EA  et al. Azithromycin decreases NALP3 mRNA stability in monocytes to limit inflammasome-dependent inflammation. Respir Res 18:131 (2017). WB ; Human . Read more (PubMed: 28659178) »
  • Sun Y  et al. Stress-induced corticotropin-releasing hormone-mediated NLRP6 inflammasome inhibition and transmissible enteritis in mice. Gastroenterology 144:1478-87, 1487.e1-8 (2013). Read more (PubMed: 23470617) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (B lymphoblastoid cells)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
200000 cells
Specification
B lymphoblastoid cells
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 28 2019

Application
Western blot
Sample
Rat Cell lysate - whole cell (Peripheral Blood Mononuclear Cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
10 ug/ml azithromycin
Specification
Peripheral Blood Mononuclear Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Apr 27 2017

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