Anti-NLRX1 antibody (ab105412)
Key features and details
- Rabbit polyclonal to NLRX1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
-
Product name
Anti-NLRX1 antibody
See all NLRX1 primary antibodies -
Description
Rabbit polyclonal to NLRX1 -
Host species
Rabbit -
Specificity
At least two isoforms of NLRX1 are known to exist; ab105412 will recognize both. This antibody is predicted to not crossreact with other NLRP family members. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIHC-P RatWB Rat
-
Immunogen
A 14 amino acid peptide near the amino terminus of Human NLRX1 (NP_078894).
-
Positive control
- Rat spleen tissue lysate
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Rat
|
| IHC-P |
Rat
|
| WB |
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
|
|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 108 kDa.
|
|
| IHC-P |
Use a concentration of 10 µg/ml.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 20 µg/ml. |
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 108 kDa. |
|
IHC-P
Use a concentration of 10 µg/ml. |
Target
-
Function
Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF-Kappa-B and type 1 interferon signaling pathways, but enhances NF-Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313). -
Tissue specificity
Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HELA, 293T, THP-1, HL60, RAJI, and JURKAT cell lines (at protein level). -
Sequence similarities
Belongs to the NLRP family.
Contains 7 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
-
Database links
- Entrez Gene: 315599 Rat
- SwissProt: Q5FVQ8 Rat
- Unigene: 12444 Rat
-
Alternative names
- Caterpiller protein 11.3 antibody
- CLR11.3 antibody
- DLNB26 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-NLRX1 antibody (ab105412)
Immunofluorescence of NLRX1 in rat spleen tissue with ab105412 at 20 ug/mL.
-
Western blot - Anti-NLRX1 antibody (ab105412)Lane 1 : Anti-NLRX1 antibody (ab105412) at 1 µg/ml
Lane 2 : Anti-NLRX1 antibody (ab105412) at 2 µg/ml
All lanes : rat spleen tissue cell lysate
Predicted band size: 108 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRX1 antibody (ab105412)Immunohistochemistry of NLRX1 in rat spleen tissue with ab105412 at 10 µg/ml.
Protocols
References (1)
ab105412 has been referenced in 1 publication.
- Peng L et al. DJ-1 exerts anti-inflammatory effects and regulates NLRX1-TRAF6 via SHP-1 in stroke. J Neuroinflammation 17:81 (2020). PubMed: 32151250
