Key features and details
- Rabbit polyclonal to NLRX1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Horse, Human
- Isotype: IgG
Product nameAnti-NLRX1 antibody
See all NLRX1 primary antibodies
DescriptionRabbit polyclonal to NLRX1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Horse, Human
Predicted to work with: Cow, Dog
Synthetic peptide, corresponding to a region within amino acids 580-630 of Human NLRX1 (NP_078894.2).
- Mouse heart tissue lysate; Human brain tissue
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab107611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 108 kDa.|
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionParticipates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF-Kappa-B and type 1 interferon signaling pathways, but enhances NF-Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313).
Tissue specificityUbiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HELA, 293T, THP-1, HL60, RAJI, and JURKAT cell lines (at protein level).
Sequence similaritiesBelongs to the NLRP family.
Contains 7 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- Caterpiller protein 11.3 antibody
- CLR11.3 antibody
- DLNB26 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human heart tissue labelling NLRX1 with ab107611 at 10µg/ml. Staining was enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
All lanes : Anti-NLRX1 antibody (ab107611) at 5 µg/ml
Lane 1 : Mouse heart tissue lysate in absence immunizing peptide
Lane 2 : Mouse heart tissue lysate in presence of immunuzing peptide
Lysates/proteins at 40 µg per lane.
All lanes : Goat anti-Rabbit Ig HRP
Developed using the ECL technique.
Predicted band size: 108 kDa
ab107611, at 5µg, staining NLRX1 in formalin-fixed, paraffin-embedded Human brain tissue by immunohistochemisty.
ab107611 has been referenced in 1 publication.
- Stokman G et al. NLRX1 dampens oxidative stress and apoptosis in tissue injury via control of mitochondrial activity. J Exp Med 214:2405-2420 (2017). PubMed: 28626071