Product nameAnti-NLRX1 antibody
See all NLRX1 primary antibodies
DescriptionRabbit polyclonal to NLRX1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Cow, Dog
Synthetic peptide corresponding to Human NLRX1 aa 900 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following lysates: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate; HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate; THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab91659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 mg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 108 kDa).|
FunctionParticipates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF-Kappa-B and type 1 interferon signaling pathways, but enhances NF-Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313).
Tissue specificityUbiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HELA, 293T, THP-1, HL60, RAJI, and JURKAT cell lines (at protein level).
Sequence similaritiesBelongs to the NLRP family.
Contains 7 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- Caterpiller protein 11.3 antibody
- CLR11.3 antibody
- DLNB26 antibody
All lanes : Anti-NLRX1 antibody (ab91659) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 108 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
ab91659 has not yet been referenced specifically in any publications.