Overview

  • Product name
    Anti-NMD3 antibody [EPR11979(B)]
    See all NMD3 primary antibodies
  • Description
    Rabbit monoclonal [EPR11979(B)] to NMD3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NMD3 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96D46

  • Positive control
    • HepG2, T47-D, Jurkat and HeLa cell lysates; Human breast and ovarian carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 58 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.
    • Sequence similarities
      Belongs to the NMD3 family.
    • Cellular localization
      Cytoplasm. Nucleus. Shuttles between the nucleus/nucleolus and the cytoplasm in a XPO1/CRM1-dependent manner.
    • Information by UniProt
    • Database links
    • Alternative names
      • 60S ribosomal export protein NMD3 antibody
      • CGI 07 antibody
      • FLJ21053 antibody
      • hNMD3 antibody
      • NMD3 antibody
      • NMD3 homolog antibody
      • NMD3 homolog (S. cerevisiae) antibody
      • NMD3 ribosome export adaptor antibody
      • NMD3, S. cerevisiae, homolog of antibody
      • NMD3_HUMAN antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (Lane 1) or 1X PBS (negative control)  (Lane 2) using ab170898 and HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

       

      Lane 1 Primary: ab170898, 1/10000 dilution
      Sample: Immunoprecipitation pellet from HeLa cell lysate
      Secondary: HRP-conjugated anti-rabbit IgG

       

      Lane 2 Primary: ab170898, 1/10000 dilution
      Sample: 1X PBS (negative control)
      Secondary: HRP-conjugated anti-rabbit IgG

       

       

       

    • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling NMD3 with ab170898 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling NMD3 with ab170898 at 1/50 dilution.

    • All lanes : Anti-NMD3 antibody [EPR11979(B)] (ab170898) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : T47-D cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 58 kDa

    References

    ab170898 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170898.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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