NMDA, excitotoxic amino acid (ab120052)
Key features and details
- Excitotoxic amino acid
- CAS Number: 6384-92-5
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
NMDA, excitotoxic amino acid -
Description
Excitotoxic amino acid -
Biological description
Excitotoxic amino acid. Prototypic agonist at the ionotropic NMDA glutamate receptor which is involved in long-term potentiation, ischemia, and epilepsy. Also available in Kit: Ionotropic agonists (ab120323).
Also available in simple stock solutions (ab146698) - add 1 ml of water to get an exact, ready-to-use concentration. -
CAS Number
6384-92-5 -
Chemical structure
Properties
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Chemical name
(R)-2-(Methylamino)succinic acid -
Molecular weight
147.13 -
Molecular formula
C5H9NO4 -
PubChem identifier
22880 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
OC(=O)C[C@@H](NC)C(=O)O -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - NMDA, excitotoxic amino acid (ab120052)2D chemical structure image of ab120052, NMDA, excitotoxic amino acid
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Functional Studies - NMDA, excitotoxic amino acid (ab120052)Sims et al PLoS One. 2013;8(1):e53814. doi: 10.1371/journal.pone.0053814. Epub 2013 Jan 11. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Release of adenosine by depolarisation and agonists.
(Panel c) NMDA application also evoked neuronal depolarisation and firing accompanied by subsequent release of adenosine and inosine.
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Functional Studies - NMDA, excitotoxic amino acid (ab120052)ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with NMDA (ab120052), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of NMDA, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120052 (NMDA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (16)
ab120052 has been referenced in 16 publications.
- Bowers MS et al. NYX-2925 induces metabotropic N-methyl-d-aspartate receptor (NMDAR) signaling that enhances synaptic NMDAR and a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor. J Neurochem 152:523-541 (2020). PubMed: 31376158
- Kiragasi B et al. The auxiliary glutamate receptor subunit dSol-1 promotes presynaptic neurotransmitter release and homeostatic potentiation. Proc Natl Acad Sci U S A 117:25830-25839 (2020). PubMed: 32973097
- Girling KD et al. Activation of caspase-6 and cleavage of caspase-6 substrates is an early event in NMDA receptor-mediated excitotoxicity. J Neurosci Res 96:391-406 (2018). PubMed: 29193273
- Kiragasi B et al. A Presynaptic Glutamate Receptor Subunit Confers Robustness to Neurotransmission and Homeostatic Potentiation. Cell Rep 19:2694-2706 (2017). Other ; Drosophila melanogaster . PubMed: 28658618
- Thaney VE et al. IFNß Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury. Sci Rep 7:46514 (2017). PubMed: 28425451