NMDA, excitotoxic amino acid (ab120052)

Submit a review Q&A (1)References (14)

Overview

  • Product name

    NMDA, excitotoxic amino acid

  • Description

    Excitotoxic amino acid

  • Biological description

    Excitotoxic amino acid. Prototypic agonist at the ionotropic NMDA glutamate receptor which is involved in long-term potentiation, ischemia, and epilepsy. Also available in Kit: Ionotropic agonists (ab120323).

    Also available in simple stock solutions (ab146698) - add 1 ml of water to get an exact, ready-to-use concentration.

  • Purity

    > 99%

  • CAS Number

    6384-92-5

  • Chemical structure

    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab120052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Functional Studies - NMDA, excitotoxic amino acid (ab120052)
    Functional Studies - NMDA, excitotoxic amino acid (ab120052)Sims et al PLoS One. 2013;8(1):e53814. doi: 10.1371/journal.pone.0053814. Epub 2013 Jan 11. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Release of adenosine by depolarisation and agonists.

    (Panel c) NMDA application also evoked neuronal depolarisation and firing accompanied by subsequent release of adenosine and inosine.

  • Functional Studies - NMDA, excitotoxic amino acid (ab120052)
    Functional Studies - NMDA, excitotoxic amino acid (ab120052)
    ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with NMDA (ab120052), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of NMDA, as described in literature.
    The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120052 (NMDA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab120052? Please let us know so that we can cite the reference in this datasheet.

ab120052 has been referenced in 14 publications.

  • Girling KD  et al. Activation of caspase-6 and cleavage of caspase-6 substrates is an early event in NMDA receptor-mediated excitotoxicity. J Neurosci Res 96:391-406 (2018). PubMed: 29193273
  • Kiragasi B  et al. A Presynaptic Glutamate Receptor Subunit Confers Robustness to Neurotransmission and Homeostatic Potentiation. Cell Rep 19:2694-2706 (2017). Other ; Drosophila melanogaster . PubMed: 28658618
  • Thaney VE  et al. IFNß Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury. Sci Rep 7:46514 (2017). PubMed: 28425451
  • Sanchez AB  et al. Antiretrovirals, Methamphetamine, and HIV-1 Envelope Protein gp120 Compromise Neuronal Energy Homeostasis in Association with Various Degrees of Synaptic and Neuritic Damage. Antimicrob Agents Chemother 60:168-79 (2016). PubMed: 26482305
  • Grosser S  et al. Hilar somatostatin interneurons contribute to synchronized GABA activity in an in vitro epilepsy model. PLoS One 9:e86250 (2014). PubMed: 24465989
View all Publications for this product

Customer reviews and Q&As

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Question

To whom it may concern, We use NMDA to induce a cognitive deficit in an passive avoidance task in rat. I have used NMDA from sigma-aldrich (product code XXXX) before which is in a free aminoacid form. Now we are trying your NMDA which is in a hemihydrate form (ab120052) and we have some problems. First it is hard to disollute (15 min in ultrasonic bath, 42 mg/1ml water) in comparsion to the sigmas NMDA. Second, the rats behave different. Abcam NMDA seems to be stronger and some rats have epileptoformic seizures after systemic administration (42 mg/kg i.p.). We have observed also some mortality, approx. 10 %. With Sigma NMDA we did not observe any seizures or mortality. Do you have any idea why it is happening ? May I kindly ask you for NMR spectra of this batch ? with best regards

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Answer

Thank you for contacting us.
I am sorry to hear you are not observing the expected results with the reagent supplied.
It is possible that ab120052 acts more potently due to it having a higher purity when compared to Sigma’s. I have attached the NMP spectra for ab120052. If you had Sigma’s NMR spectra for this product, we would be happy to compare it against ours to determine if this is the case.
It is also worth considering that variations in the strength of the epileptoformic seizures can occur due to variations in batches of rats.
We would also recommend using the batch specific molecular weight when calculating doses.
As a final note, variations in seizure strength could occur if the solvent/saline solution is not fresh or contaminated – this should be checked before carrying out experimental procedures.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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