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To whom it may concern, We use NMDA to induce a cognitive deficit in an passive avoidance task in rat. I have used NMDA from sigma-aldrich (product code XXXX) before which is in a free aminoacid form. Now we are trying your NMDA which is in a hemihydrate form (ab120052) and we have some problems. First it is hard to disollute (15 min in ultrasonic bath, 42 mg/1ml water) in comparsion to the sigmas NMDA. Second, the rats behave different. Abcam NMDA seems to be stronger and some rats have epileptoformic seizures after systemic administration (42 mg/kg i.p.). We have observed also some mortality, approx. 10 %. With Sigma NMDA we did not observe any seizures or mortality. Do you have any idea why it is happening ? May I kindly ask you for NMR spectra of this batch ? with best regards
Asked on Sep 14 2012
Thank you for contacting us.
I am sorry to hear you are not observing the expected results with the reagent supplied.
It is possible that ab120052 acts more potently due to it having a higher purity when compared to Sigma’s. I have attached the NMP spectra for ab120052. If you had Sigma’s NMR spectra for this product, we would be happy to compare it against ours to determine if this is the case.
It is also worth considering that variations in the strength of the epileptoformic seizures can occur due to variations in batches of rats.
We would also recommend using the batch specific molecular weight when calculating doses.
As a final note, variations in seizure strength could occur if the solvent/saline solution is not fresh or contaminated – this should be checked before carrying out experimental procedures.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Sep 14 2012