Anti-NMDAR1 antibody (ab254108)
Key features and details
- Rabbit polyclonal to NMDAR1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NMDAR1 antibody
See all NMDAR1 primary antibodies -
Description
Rabbit polyclonal to NMDAR1 -
Host species
Rabbit -
Specificity
Specific for endogenous levels of the ~120 kDa NR1 subunit of the NMDA receptor containing the C1 splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C1 insert.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Dog, Non human primates
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Immunogen
Synthetic peptide within NMDAR1. The exact sequence is proprietary. Peptide from the NR1 subunit.
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Positive control
- WB: HEK-293 expressing the NMDAR NR1 subunit, C1 splice variant insert.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 105 kDa.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 105 kDa. |
Target
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. -
Post-translational
modificationsNMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities. - Information by UniProt
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Database links
- Entrez Gene: 494007 Dog
- Entrez Gene: 2902 Human
- Entrez Gene: 14810 Mouse
- Omim: 138249 Human
- SwissProt: Q5R1P0 Dog
- SwissProt: Q05586 Human
- SwissProt: P35438 Mouse
- Unigene: 558334 Human
see all -
Alternative names
- GluN1 antibody
- Glutamate [NMDA] receptor subunit zeta-1 antibody
- Glutamate receptor ionotropic N methyl D aspartate 1 antibody
see all
Images
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Western blot - Anti-NMDAR1 antibody (ab254108)All lanes : Anti-NMDAR1 antibody (ab254108) at 1/1000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : HEK-293 expressing NR1 subunit containing only the C2 insert, cell lysate
Lane 3 : HEK-293 expressing NR1 subunit containing the C1 and C2' insert, cell lysate
Lane 4 : HEK-293 expressing NR1 subumit containg the N1 and C2' insert, cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 105 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab254108 has not yet been referenced specifically in any publications.
