Overview

  • Product name
  • Description
    Rabbit polyclonal to NMDAR1
  • Host species
    Rabbit
  • Specificity
    ab52177 detects endogenous levels of total NMDAR1 protein.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human NMDAR1 aa 849-913. The exact sequence is proprietary. From around the phosphorylation site of serine 897 (R-S-S-K-D).

  • Positive control
    • IHC-P: Human brain tissue. WB: A549, Y79 and Raji whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 105 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
  • Post-translational
    modifications
    NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
  • Cellular localization
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • Database links
  • Alternative names
    • GluN1 antibody
    • Glutamate [NMDA] receptor subunit zeta-1 antibody
    • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
    • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
    • glutamate receptor ionotropic, NMDA 1 antibody
    • Grin1 antibody
    • MRD8 antibody
    • N methyl D aspartate receptor antibody
    • N methyl D aspartate receptor channel subunit zeta 1 antibody
    • N methyl D aspartate receptor subunit NR1 antibody
    • N-methyl-D-aspartate receptor subunit NR1 antibody
    • NMD-R1 antibody
    • NMDA 1 antibody
    • NMDA R1 antibody
    • NMDA receptor 1 antibody
    • NMDA1 antibody
    • NMDAR antibody
    • NMDZ1_HUMAN antibody
    • NR1 antibody
    see all

Images

  • All lanes : Anti-NMDAR1 antibody (ab52177) at 1/500 dilution

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) whole cell lysate
    Lane 2 : Y79 (Human retinoblastoma cell line) whole cell lysate
    Lane 3 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 105 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 30 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

  • ab52177 at 1/50 dilution staining NMDAR1 in human brain tissue by immunohistochemistry (paraffin embedded tissue) in the presence (right image) or absence (left image) of the immunizing peptide.

References

This product has been referenced in:
  • Kushwah N  et al. Hypobaric Hypoxia-Induced Learning and Memory Impairment: Elucidating the Role of Small Conductance Ca2+-Activated K+ Channels. Neuroscience 388:418-429 (2018). Read more (PubMed: 30048783) »
  • Roshanravan H  et al. NMDA Receptors as Potential Therapeutic Targets in Diabetic Nephropathy: Increased Renal NMDA Receptor Subunit Expression in Akita Mice and Reduced Nephropathy Following Sustained Treatment With Memantine or MK-801. Diabetes 65:3139-50 (2016). Read more (PubMed: 27388219) »
See all 8 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Miss. Narin Ilgım Ardıç

Verified customer

Submitted May 09 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Kidney)
Specification
Kidney
Permeabilization
No
Fixative
Paraformaldehyde

Mrs. Lola Sanchez

Verified customer

Submitted Feb 26 2015

Answer

Thanks for your email.

I have located your order. I am happy to send a vial of anti-GFAP antibody ab53554 as the replacement. We do have this product in stock and I will ship it Monday for Tuesday delivery. I will send you an email containing the order reference number for this replacement order today, for your records.

Also, I'm afraid our order processing system is not designed to handle your request to combine this replacement order with an upcoming order from your lab.

I hope this is helpful. Please contact me again if you have any further questions.

Read More

Answer

Thanks for your email.

I did see the western blot image that you kindly attached to your last email. There are a few bands found in the "No Primary Ab" blot but not nearly as many as can be seen in the blot on the left which includes the primary. In the blot on the left it is clear that many non specific bands are being detected by the primary antibody. There is no way to determine which band is NMDAR1 based on the data you have provided.

This problem does sometimes occur with antibodies. It is for cases like this that we have a guarantee which essentially states that if the antibody is not performing as expected based on the online antibody datasheet, we are happy to provide a replacement of any primary antibody, credit or refund, as long as the customer follows up with us within 6 months of receiving the product.

I believe in this case the vial of antibody you received is not working as expected.

Again, Iam sorry this antibody is giving you trouble. I am happy to offer a replacement of any primary antibody, credit or refund. If you are interested, please let me know how you wish to proceed. In your reply, kindly include the PO number or Abcam order reference number associated with this purchase of ab52177 so I can process your request.

Read More

Answer

Thanks for your email.

I am sorry this antibody is giving you trouble. I am happy to offer a replacement of any primary antibody, credit or refund. If you are interested, please let me know how you wish to proceed. In your reply, kindly include the PO number or Abcam order reference number associated with this purchase of ab52177 so I can process your request.

Read More

Answer

Thanks for your reply.

For a negative control, I was thinking of samples in which you do not expect to see NMDAR1 expression. Since there is more than one bandinyour sample lanesI think it would be helpful to compare a sample where there is definitely no NMDAR1 expression.

A "no primary" control is a good idea too to determine if any of the bands you are seeing are due to non-specific interactions of the secondary antibody. But if this secondary antibody has been recently used with other primary antibodies with expected results, then this "no primary" control is probably not necessary.

I hope this is helpful. Please contact me again if you have any further questions.

Read More

Question
Answer

Thanks so much for all your troubleshooting efforts!

Do the gels from your colleague your the gel image you sent on Sunday contain any negative controls samples, ie. samples in which you do not expect to see NMDAR1 expression? If so, please note which lanes are the negative control lanes. Thanks!

Read More

Answer

Thanks for your enquiry and for including the western blot image.

It does not appear that NMDAR1 is being detectedon your western blot. I just have a couple additional questions:

1. How much protein was loaded per lane on the western blot?

2. What dilutions of the primary were tested?

3. Were any other antibodies used successfully to blot these same tissues?

Thanks in advance for the additional information!

Read More

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