Recombinant Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2481(2)] to NMDAR1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NMDAR1 antibody [EPR2481(2)]
See all NMDAR1 primary antibodies -
Description
Rabbit monoclonal [EPR2481(2)] to NMDAR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal brain cell lysate. ICC/IF: Mouse primary neuron cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2481(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 105 kDa).
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ICC/IF |
1/50.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 105 kDa). |
ICC/IF
1/50. |
Target
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. -
Post-translational
modificationsNMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities. - Information by UniProt
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Database links
- Entrez Gene: 2902 Human
- Entrez Gene: 14810 Mouse
- Entrez Gene: 24408 Rat
- Omim: 138249 Human
- SwissProt: Q05586 Human
- SwissProt: P35438 Mouse
- SwissProt: P35439 Rat
- Unigene: 558334 Human
see all -
Alternative names
- GluN1 antibody
- Glutamate [NMDA] receptor subunit zeta-1 antibody
- Glutamate receptor ionotropic N methyl D aspartate 1 antibody
see all
Images
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All lanes : Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/5000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 105 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling NMDAR1 with purified ab109182 at 1/50 (9.5µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. -
Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/1000 dilution (purified) + Human cerebellum tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 105 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 105 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/1000 dilution (unpurified) + Human fetal brain cell lysate at 10 µg
Predicted band size: 105 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Secondary antibody - anti-rabbit HRP (ab6721)
Datasheets and documents
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SDS download
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Datasheet download
References (47)
ab109182 has been referenced in 47 publications.
- Kim YJ et al. An autophagy-related protein Becn2 regulates cocaine reward behaviors in the dopaminergic system. Sci Adv 7:N/A (2021). PubMed: 33608268
- Loera-Valencia R et al. High levels of 27-hydroxycholesterol results in synaptic plasticity alterations in the hippocampus. Sci Rep 11:3736 (2021). PubMed: 33580102
- Imbriglio T et al. Developmental up-regulation of NMDA receptors in the prefrontal cortex and hippocampus of mGlu5 receptor knock-out mice. Mol Brain 14:77 (2021). PubMed: 33962661
- Verhaeghe R et al. Maternal stress programs a demasculinization of glutamatergic transmission in stress-related brain regions of aged rats. Geroscience N/A:N/A (2021). PubMed: 33983623
- Chen M et al. Enhancing GABAergic signaling ameliorates aberrant gamma oscillations of olfactory bulb in AD mouse models. Mol Neurodegener 16:14 (2021). PubMed: 33663578