Recombinant
RabMAb

Recombinant Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

Reviews (4)Q&A (5)References (27)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2481(2)] to NMDAR1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NMDAR1 antibody [EPR2481(2)]
    See all NMDAR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2481(2)] to NMDAR1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human NMDAR1 aa 850-950. The exact sequence is proprietary.
    Database link: Q05586

  • Positive control

    • Fetal brain cell lysate. ICC/IF: Mouse primary neuron cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab109182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 105 kDa).
ICC/IF 1/50.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.

    • Sequence similarities

      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.

    • Post-translational
      modifications

      NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.

    • Cellular localization

      Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • GluN1 antibody
      • Glutamate [NMDA] receptor subunit zeta-1 antibody
      • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
      • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
      • glutamate receptor ionotropic, NMDA 1 antibody
      • Grin1 antibody
      • MRD8 antibody
      • N methyl D aspartate receptor antibody
      • N methyl D aspartate receptor channel subunit zeta 1 antibody
      • N methyl D aspartate receptor subunit NR1 antibody
      • N-methyl-D-aspartate receptor subunit NR1 antibody
      • NMD-R1 antibody
      • NMDA 1 antibody
      • NMDA R1 antibody
      • NMDA receptor 1 antibody
      • NMDA1 antibody
      • NMDAR antibody
      • NMDZ1_HUMAN antibody
      • NR1 antibody
      see all

    Images

    • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      All lanes : Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/5000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 105 kDa
      Observed band size: 120 kDa
      why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Immunocytochemistry/ Immunofluorescence - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

      Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling NMDAR1 with purified ab109182 at 1/50 (9.5µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
      Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.

    • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/1000 dilution (purified) + Human cerebellum tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 105 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 105 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/1000 dilution (unpurified) + Human fetal brain cell lysate at 10 µg

      Predicted band size: 105 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?



      Secondary antibody - anti-rabbit HRP (ab6721)

    References (27)

    Publishing research using ab109182? Please let us know so that we can cite the reference in this datasheet.

    ab109182 has been referenced in 27 publications.

    • Hudry E  et al. Opposing Roles of apolipoprotein E in aging and neurodegeneration. Life Sci Alliance 2:N/A (2019). PubMed: 30760557
    • Tabassum S  et al. Jujuboside A prevents sleep loss-induced disturbance of hippocampal neuronal excitability and memory impairment in young APP/PS1 mice. Sci Rep 9:4512 (2019). PubMed: 30872728
    • Li J  et al. Mechanisms of Aerobic Exercise Upregulating the Expression of Hippocampal Synaptic Plasticity-Associated Proteins in Diabetic Rats. Neural Plast 2019:7920540 (2019). PubMed: 30911292
    • Wang H  et al. Prenatal Exposure to Antipsychotics Disrupts the Plasticity of Dentate Neurons and Memory in Adult Male Mice. Int J Neuropsychopharmacol 22:71-82 (2019). PubMed: 30169628
    • Zheng S  et al. Exercise training increases spatial memory via reducing contralateral hippocampal NMDAR subunits expression in intracerebral hemorrhage rats. Neuropsychiatr Dis Treat 15:1921-1928 (2019). PubMed: 31371965
    View all Publications for this product

    Customer reviews and Q&As

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    1-9 of 9 Abreviews or Q&A

    Western blot abreview for Anti-NMDAR1 antibody [EPR2481(2)]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 01 2019

    Western blot abreview for Anti-NMDAR1 antibody [EPR2481(2)]

     Below Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Monkey Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 01 2019

    Western blot abreview for Anti-NMDAR1 antibody [EPR2481(2)]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 01 2019

    Question

    Thank you for your e-mail. Yes, the information helped a lot. Based on the sequence I believe this corresponds to a region, which varies between NMDAR1 subunit isoforms (according to a quick Uniprot search).

    Read More
    Answer

    That is correct, according to Uniprot the exact immunogen sequence is missing in isoforms 1, 2 and 7 but conserved in isoforms 3 (also known as long, NR-1-3), 4, 5 and 6.

    Read More

    Western blot abreview for Anti-NMDAR1 antibody [EPR2481(2)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
    Sample
    Rat Tissue lysate - other (hippocampi total membrane fraction)
    Specification
    hippocampi total membrane fraction
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Ms. Sunetra Jitkar

    Verified customer

    Submitted Feb 19 2014

    Question

    Do you have a detailed protocol of flow cytometric analysis of permeabilized SH-SY5Y cells which are demonstrated in the datasheet?

    Read More
    Answer

    The protocol used was our standard FC protocol with ab109182 Anti-NMDAR1 antibody [EPR2481(2)]. Please see the link below for more details.

    http://www.epitomics.com/products/support/fc

    Read More

    Question

    Can I use this antibody with live cells?

    Read More
    Answer

    Thank you for your enquiry.

    Unfortunately, we do not have the epitope information for this antibody but according to the immunogen sequence, it is towards the cytoplasmic region of the membrane. Therefore, this product may not be suitable for live cells in flow cytometry.

    I hope this information will be helpful.

    Read More

    Question

    Can I use this product with live cells?

    Read More
    Answer

    Thank you for your enquiry.

    This product was tested with permeabilized SH‐SY5Y cells and the cells were fixed with 2% paraformaldehyde and incubated at room temperature for 10 min. Our labs use the intracellular staining protocol only, and so there is no guarantee if this antibody can be used with live cells.

    In general, it should not be a problem but should you not see expected results, please try with fixed cells.

    I hope this information will be helpful.

    Read More

    Question

    I have found an antibody from another company called "rabbit anti-NR1pan" in the paper : "Newborn- and adult-derived brain microvascular endothelial cells show age-related differences in phenotype and glutamate-evoked protease release" by Legros et al. in Journal of Cerebral Blood Flow & Metabolism (2009) 29, 1146-1158. Unfortuneately, the company stopped selling this antibody.

    Thus, I wanted to ask whether you can offer a good primary antibody against the NR1 subunit of the NMDAR (NMDARNR1, Grin1, NMDAR1) for western blotting and immunofluorescence. This antibody has to react with mouse and recognize the isoforms of NR1 that means it should be a PAN-antibody, the preferred species would be rabbit.

    Read More
    Answer

    Thank you for contacting us.

    We have a few rabbit-raised antibodies to NMDAR1 that were tested in mouse as well as WB and ICC.

    ab28669 (https://www.abcam.com/NMDAR1-antibody-ab28669.html), but based on the immunogen sequence, it would only recognize only isoform 1 in mouse.

    ab109182 (https://www.abcam.com/NMDAR1-antibody-EPR2481-2-ab109182.html). Here I currently have no detailed information about the location of the immunogen peptide, and will inquire with the lab about that. I will let you know when I hear back from them.

    ab17345 (https://www.abcam.com/NMDAR1-antibody-Neuronal-Marker-ab17345.html). This antibody was raised against a peptide in the N-terminal region which seems to be conserved among the isoforms. The antibody has not yet been tested inmouse, but is predicted to react based on sequence homology. In that case I can offer you a testing discount code for trying it in mouse. Please see for more information below.

    We guarantee all our products for tested species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.

    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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