Recombinant
RabMAb

Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

Abreviews (1)Q&A (5)Specific References (11)

Overview

  • Product name
    Anti-NMDAR1 antibody [EPR2481(2)]
    See all NMDAR1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2481(2)] to NMDAR1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NMDAR1 aa 850-950. The exact sequence is proprietary.

  • Positive control
    • Fetal brain cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 105 kDa).
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function
      NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
    • Sequence similarities
      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
    • Post-translational
      modifications
      NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
    • Cellular localization
      Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • GluN1 antibody
      • Glutamate [NMDA] receptor subunit zeta-1 antibody
      • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
      • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
      • glutamate receptor ionotropic, NMDA 1 antibody
      • Grin1 antibody
      • MRD8 antibody
      • N methyl D aspartate receptor antibody
      • N methyl D aspartate receptor channel subunit zeta 1 antibody
      • N methyl D aspartate receptor subunit NR1 antibody
      • N-methyl-D-aspartate receptor subunit NR1 antibody
      • NMD-R1 antibody
      • NMDA 1 antibody
      • NMDA R1 antibody
      • NMDA receptor 1 antibody
      • NMDA1 antibody
      • NMDAR antibody
      • NMDZ1_HUMAN antibody
      • NR1 antibody
      see all

    Images

    • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      All lanes : Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/5000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 105 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/1000 dilution (purified) + Human cerebellum tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 105 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 105 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)
      Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/1000 dilution (unpurified) + Human fetal brain cell lysate at 10 µg

      Predicted band size: 105 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)



      Secondary antibody - anti-rabbit HRP (ab6721)

    References

    This product has been referenced in:
    • Li J  et al. Differential Behavioral and Neurobiological Effects of Chronic Corticosterone Treatment in Adolescent and Adult Rats. Front Mol Neurosci 10:25 (2017). WB . Read more (PubMed: 28210212) »
    • Liu E  et al. GSK-3ß deletion in dentate gyrus excitatory neuron impairs synaptic plasticity and memory. Sci Rep 7:5781 (2017). Read more (PubMed: 28720858) »

    See all 11 Publications for this product

    Publishing research using ab109182? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    6 reviews or Q&A

    10
    Votes: Highest First

    Thank you for your e-mail. Yes, the information helped a lot. Based on the sequence I believe this corresponds to a region, which varies between NMDAR1 subunit isoforms (according to a quick Uniprot search).

    That is correct, according to Uniprot the exact immunogen sequence is missing in isoforms 1, 2 and 7 but conserved in isoforms 3 (also known as long, NR-1-3), 4, 5 and 6.

    Western blot abreview for Anti-NMDAR1 antibody [EPR2481(2)]

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
    Sample
    Rat Tissue lysate - other (hippocampi total membrane fraction)
    Specification
    hippocampi total membrane fraction
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    abreview image
    Username

    Ms. Sunetra Jitkar

    Verified customer

    Submitted Feb 19 2014

    Do you have a detailed protocol of flow cytometric analysis of permeabilized SH-SY5Y cells which are demonstrated in the datasheet?

    The protocol used was our standard FC protocol with ab109182 Anti-NMDAR1 antibody [EPR2481(2)]. Please see the link below for more details.

    http://www.epitomics.com/products/support/fc

    Can I use this antibody with live cells?

    Thank you for your enquiry.

    Unfortunately, we do not have the epitope information for this antibody but according to the immunogen sequence, it is towards the cytoplasmic region of the membrane. Therefore, this product may not be suitable fo...

    Read More

    Can I use this product with live cells?

    Thank you for your enquiry.

    This product was tested with permeabilized SH‐SY5Y cells and the cells were fixed with 2% paraformaldehyde and incubated at room temperature for 10 min. Our labs use the intracellular staining protocol only, and s...

    Read More

    I have found an antibody from another company called "rabbit anti-NR1pan" in the paper : "Newborn- and adult-derived brain microvascular endothelial cells show age-related differences in phenotype and glutamate-evoked protease release" by Legros et al....

    Read More

    Thank you for contacting us.

    We have a few rabbit-raised antibodies to NMDAR1 that were tested in mouse as well as WB and ICC.

    ab28669 (https://www.abcam.com/NMDAR1-antibody-ab28669.html), but based on the immunogen sequence, it wo...

    Read More

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