Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
Sample
Rat Tissue lysate - other (hippocampi total membrane fraction)
Specification
hippocampi total membrane fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
19 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% blocking milk in 0.1% TBST
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 1200, 610, 750 kDa
Exposure
1 minute(s) and 40 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Sunetra Jitkar
Verified customer
Submitted Feb 19 2014