Anti-NMDAR1 antibody [N308/48] - Neuronal Marker (ab134308)
Key features and details
- Mouse monoclonal [N308/48] to NMDAR1 - Neuronal Marker
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-NMDAR1 antibody [N308/48] - Neuronal Marker
See all NMDAR1 primary antibodies -
Description
Mouse monoclonal [N308/48] to NMDAR1 - Neuronal Marker -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Fusion protein corresponding to Rat NMDAR1 aa 1-400 (Hinge).
Database link: P35439 -
Positive control
- WB: Human, Mouse and Rat brain membrane tissue lysate, Neuro-2a cell lysate. ICC/IF: SK-N-BE cells. Flow Cyt: SH-SY5Y cells.
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General notes
The clone number has been updated from S308-48 to N308/48, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N308/48 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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WB |
1/1000. Predicted molecular weight: 105 kDa.
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ICC/IF |
1/100.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 105 kDa. |
ICC/IF
1/100. |
Target
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. -
Post-translational
modificationsNMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities. - Information by UniProt
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Database links
- Entrez Gene: 2902 Human
- Entrez Gene: 14810 Mouse
- Entrez Gene: 24408 Rat
- Omim: 138249 Human
- SwissProt: Q05586 Human
- SwissProt: P35438 Mouse
- SwissProt: P35439 Rat
- Unigene: 558334 Human
see all -
Alternative names
- GluN1 antibody
- Glutamate [NMDA] receptor subunit zeta-1 antibody
- Glutamate receptor ionotropic N methyl D aspartate 1 antibody
see all
Images
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All lanes : Anti-NMDAR1 antibody [N308/48] - Neuronal Marker (ab134308) at 1/1000 dilution
Lane 1 : Wild-type Neuro-2a cell lysate
Lane 2 : GRIN1 knockout Neuro-2a cell lysate
Lane 3 : SH-SY5Y UNBOILED cell lysate
Lane 4 : THP-1 UNBOILED cell lysate
Lane 5 : Human Brain UNBOILED cell lysate
Lane 6 : Human Liver UNBOILED cell lysate
Lane 7 : Mouse Brain UNBOILED cell lysate
Lane 8 : Mouse Liver UNBOILED cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 105 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Western blot: Anti-GRIN1 antibody [N308/48] (ab134308) staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab134308 was shown to bind specifically to GRIN1. A band was observed at 120 kDa in wild-type Neuro-2a cell lysates with no signal observed at this size in GRIN1 knockout cell line ab281960 (knockout cell lysate ab282987). To generate this image, unboiled wild-type and GRIN1 knockout Neuro-2a cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
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Anti-NMDAR1 antibody [N308/48] - Neuronal Marker (ab134308) at 1/1000 dilution + Rat brain membrane tissue lysate
Secondary
Goat anti-mouse IgG HRP
Predicted band size: 105 kDa -
SK-N-BE cells labeling NMDAR1 using ab134308 at 1/100 dilution in ICC/IF. Cells were fixed using 4% formaldehyde for 15 minutes at room temperature. Incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).
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Overlay histogram showing SH-SY5Y cells stained with ab134308 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab134308, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab134308 has been referenced in 12 publications.
- Crespo M et al. Na+/K+- and Mg2+-ATPases and Their Interaction with AMPA, NMDA and D2 Dopamine Receptors in an Animal Model of Febrile Seizures. Int J Mol Sci 23:N/A (2022). PubMed: 36498965
- Zhang C et al. A Safety Study of the Effects of 2-Dimensional Shear Wave Elastography on Synaptic Morphologic Characteristics and Function in the Hippocampus of Neonatal Mice. J Ultrasound Med 40:163-173 (2021). PubMed: 32681671
- Wang Y et al. mGluR5 mediates ketamine antidepressant response in susceptible rats exposed to prenatal stress. J Affect Disord 272:398-408 (2020). PubMed: 32553383
- Cheng H et al. Over-activation of NMDA receptors promotes ABCA1 degradation and foam cell formation. Biochim Biophys Acta Mol Cell Biol Lipids 1865:158778 (2020). PubMed: 32745694
- Durham RJ et al. Conformational spread and dynamics in allostery of NMDA receptors. Proc Natl Acad Sci U S A 117:3839-3847 (2020). PubMed: 32015122