Product nameAnti-NMDAR1 antibody [N308/48] (Biotin)
See all NMDAR1 primary antibodies
DescriptionMouse monoclonal [N308/48] to NMDAR1 (Biotin)
Tested applicationsSuitable for: IHC-P, WB, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Fusion protein corresponding to Rat NMDAR1 aa 42-361 (extracellular). NP_058706.1
FREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCEDLISSQVYAIL VSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTV PPYSHQSSVWFEMMRVYNWNHIILLVSDDHEGRAAQKRLETLLEERESKA EKVLQFDPGTKNVTALLMEARELEARVIILSASEDDAATVYRAAAMLNMT GSGYVWLVGEREISGNALRYAPDGIIGLQLINGKNESAHISDAVGVVAQA VHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFN EDGDRKFANYSIMNLQNRKL
Database link: P35439
- Rat brain tissue lysate
The clone number has been updated from S308-48 to N308/48, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab183434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.1 - 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa.
1 µg/ml of ab183434 is sufficient for detection of NMDAR1 in 20µg of rat brain membrane lysate.
|IHC-Fr||Use a concentration of 0.1 - 1 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionNMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
modificationsNMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
- Information by UniProt
- GluN1 antibody
- Glutamate [NMDA] receptor subunit zeta-1 antibody
- Glutamate receptor ionotropic N methyl D aspartate 1 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab183434 has not yet been referenced specifically in any publications.