• Product name
    Anti-NMDAR1 antibody - Neuronal Marker
    See all NMDAR1 primary antibodies
  • Description
    Rabbit polyclonal to NMDAR1 - Neuronal Marker
  • Host species
  • Tested applications
    Suitable for: IHC (PFA fixed), WB, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat NMDAR1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab17568.)

  • Positive control
    • Rat brain and spinal cord


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab17345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).Can be blocked with Rat NMDAR1 peptide (ab17568).
IHC-FoFr Use at an assay dependent concentration. PubMed: 20831598
ICC/IF 1/1000.


  • Function
    NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
  • Post-translational
    NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
  • Cellular localization
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • Database links
  • Alternative names
    • GluN1 antibody
    • Glutamate [NMDA] receptor subunit zeta-1 antibody
    • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
    • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
    • glutamate receptor ionotropic, NMDA 1 antibody
    • Grin1 antibody
    • MRD8 antibody
    • N methyl D aspartate receptor antibody
    • N methyl D aspartate receptor channel subunit zeta 1 antibody
    • N methyl D aspartate receptor subunit NR1 antibody
    • N-methyl-D-aspartate receptor subunit NR1 antibody
    • NMD-R1 antibody
    • NMDA 1 antibody
    • NMDA R1 antibody
    • NMDA receptor 1 antibody
    • NMDA1 antibody
    • NMDAR antibody
    • NMDZ1_HUMAN antibody
    • NR1 antibody
    see all


  • All lanes : Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/ml

    Lane 1 : HEK293 Whole Cell Lysate Transfected with SHAM
    Lane 2 : HEK293 Whole Cell Lysate Transfected with NR1-1a/NR2A

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 105 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 30 seconds

    NMDAR1 has a predicted molecular weight of 105kDa; however it has a number of potential glycosylation and phosphorylation sites which may affect the migration of the protein (SwissProt data). In addition, a band at 37kDa has been observed (data not shown).
  • ab17345 Rabbit polyclonal to NMDAR1 immunofluorescent staining in the rat brain cortex. Primary antibody ab17345 was incubated at room temperature overnight at 0.5µg/ml. Preincubation with the immunizing peptide (ab17568) resulted in complete blocking of the primary antibody staining. Anti rabbit alexa fluor 488 secondary antibody was used at 1/1000 (incubated for 2h at room temperature). Protocol details: rat tissue was intracardially perfusion fixed with 4% PFA followed by an overnight post fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. As expected NMDAR1 staining is observed in the soma, axon and dendrites of neurons.

  • Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/ml + HEK293 cell lysate co-transfected
    with NR1 and NR2A recombinant protein at 50 µg

    Predicted band size: 105 kDa
    Observed band size: ~110 kDa why is the actual band size different from the predicted?

    HEK293 cells were co-transfected with NMDAR1 (expected MW ~ 105kDa) and NMDAR2A (expected MW ~ 180kDa).  ab17345 NMDAR1 antibody specifically recognises NMDAR1 protein and does not recognise NMDAR2A protein. ab17345 was incubated overnight at 4°C at 2µg/ml.


This product has been referenced in:
  • Estrada-Rojo F  et al. Diurnal variation of NMDA receptor expression in the rat cerebral cortex is associated with traumatic brain injury damage. BMC Res Notes 11:150 (2018). Read more (PubMed: 29467028) »
  • Torres L  et al. Toxoplasma gondii alters NMDAR signaling and induces signs of Alzheimer's disease in wild-type, C57BL/6 mice. J Neuroinflammation 15:57 (2018). Read more (PubMed: 29471842) »
See all 17 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


Thank you for contacting us.

We have a few rabbit-raised antibodies to NMDAR1 that were tested in mouse as well as WB and ICC.

ab28669 (https://www.abcam.com/NMDAR1-antibody-ab28669.html), but based on the immunogen sequence, it would only recognize only isoform 1 in mouse.

ab109182 (https://www.abcam.com/NMDAR1-antibody-EPR2481-2-ab109182.html). Here I currently have no detailed information about the location of the immunogen peptide, and will inquire with the lab about that. I will let you know when I hear back from them.

ab17345 (https://www.abcam.com/NMDAR1-antibody-Neuronal-Marker-ab17345.html). This antibody was raised against a peptide in the N-terminal region which seems to be conserved among the isoforms. The antibody has not yet been tested inmouse, but is predicted to react based on sequence homology. In that case I can offer you a testing discount code for trying it in mouse. Please see for more information below.

We guarantee all our products for tested species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Immunocytochemistry/ Immunofluorescence
Rat Cell (Primary Neurons Cortex)
Primary Neurons Cortex
Yes - Methanol
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Apr 11 2008

Immunocytochemistry/ Immunofluorescence
Rat Cell (Brain)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 07 2007

Immunohistochemistry (Frozen sections)
Rat Tissue sections (hippocampus and cortex)
hippocampus and cortex

Dr. Sophie Pezet

Verified customer

Submitted Apr 12 2006

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